ABSTRACT
Piper guineense (Schumach) has great potentials for economic exploration because of the
proven use of its medicinal content in human health. The plant material is usually
obtained from thick forests by women who have to walk long distances in the bush to
reach it. A technique that improves its propagation and domestication, such as tissue
culture, becomes necessary. This study was therefore initiated to develop a method for
the mass propagation of Piper guineense seedlings using in vitro regeneration. Plants
react differently to media concentrations and constituents for their in vitro regeneration.
Comparative growth of Piper guneensee inoculated on Murashige and Skoog(MS)
medium supplemented with some growth regulators were investigated. Mature nodes
were collected from the medicinal garden of National Centre for Genetic Resources and
Biotechnology (NACGRAB) Ibadan. Nodes of the species were inoculated onto MS
media. The MS media was supplemented with the following concentrations of growth
regulators 0.25, 0.50, 0.75, 1.0mg/l. BAP, the same concentration for KIN and 0.05mg/l
of NAA for all replicate. After two weeks of inoculation it was observed that, the plant let
with BAP 0.50mg/l +NAA 0.05mg/l were found contamination-free and sprouted, while
other treatments showed contamination including control. Furthermore, after 7 days the
sprouted, plantlets with treatment of BAP 0.50mg/l +NAA 0.05mg/l were also
contaminated, thereby yielding no positive result. Many repeats of the experiment also yielded contaminated products
Tope, O (2021). Establishing A Method For The Mass Propagation Of Piper Guineense (Schumach) Via Tissue Culture. Afribary. Retrieved from https://track.afribary.com/works/establishing-a-method-for-the-mass-propagation-of-piper-guineense-schumach-via-tissue-culture
Tope, OKAFOR "Establishing A Method For The Mass Propagation Of Piper Guineense (Schumach) Via Tissue Culture" Afribary. Afribary, 21 May. 2021, https://track.afribary.com/works/establishing-a-method-for-the-mass-propagation-of-piper-guineense-schumach-via-tissue-culture. Accessed 27 Nov. 2024.
Tope, OKAFOR . "Establishing A Method For The Mass Propagation Of Piper Guineense (Schumach) Via Tissue Culture". Afribary, Afribary, 21 May. 2021. Web. 27 Nov. 2024. < https://track.afribary.com/works/establishing-a-method-for-the-mass-propagation-of-piper-guineense-schumach-via-tissue-culture >.
Tope, OKAFOR . "Establishing A Method For The Mass Propagation Of Piper Guineense (Schumach) Via Tissue Culture" Afribary (2021). Accessed November 27, 2024. https://track.afribary.com/works/establishing-a-method-for-the-mass-propagation-of-piper-guineense-schumach-via-tissue-culture