Current method of isolating pure culture

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ABSTRACT

Pure Culture is a laboratory Culture that contains a single species of organisms.We have existing and current method of isolating pure culture which are spread plate, streak plate, pour plate and serial dilution this existing onces uses agar plate plate and is manually done and incubated for three days. but the current methods which are the capillary pipette, fluorescence activated sorting, laser capture microdissection etc they uses machine for its isolation. There are differences between the current and existing ,the current current are very fast to sort out single cell but the existing takes three days before it obtain a single organism.

TABLE OF CONTENT

Dedication

Acknowledgment

Table of content

Abstract

SECTION ONE

1.0 Introduction

1.1 Pure Culture

1.2 Exiting methods of isolating pure cultures

1.3 Isolation of pure culture by streak plate method

1.4 Isolation of pure culture by pour plate method

1.5 Isolation of pure culture by spread plate method

1.6 Isolation of pure culture by serial dilution method

SECTION TWO

2.0 Current methods of isolating pure cultures

2.1 Flouresecence Activated Cell Sorting (FACS)

2.2 Optical Tweezers

2.3 Capillary pipette

2.4 Micromanipulator System

2.5 Laser Capture Micro Dissection

SECTION THREE

3.0 Bactotip

3.1 Limiting Dilution

3.2 Microfluidics

3.3 Manual Cell Picking

3.4 Features of Discussed technologies

3.5 Differences between Existing Methods and Current Methods

SECTION FOUR

Conclusion

Reference

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Current method of isolating pure culture

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