Abstract/Overview
Clinical immunity to malaria declines in the absence of repeated parasite exposure. However, little is known about how B cell populations and antigen-specific memory B cells change in the absence of P. falciparum infection. A successful indoor residual insecticide spraying campaign in a highland area of western Kenya, led to an absence of blood-stage P. falciparum infection between March 2007 and April 2008. We assessed memory B cell responses in 45 adults at the beginning (April2008) and end (April 2009) of a subsequent 12-month period during which none of the adults had evidence of asymptomatic parasitemia or clinical disease. Antibodies and memory B cells to the 42-kDa portion of the merozoite surface protein-1 (MSP-142) were measured using ELISA and ELISPOT assays, respectively. B cell populations were characterized by flow cytometry. From 2008 to 2009, the prevalence of MSP-142-specific memory B cells (45% vs. 55%, respectively, P = 0.32) or antibodies (91% vs. 82%, respectively, P = 0.32) did not differ significantly, although specific individuals did change from positive to negative and vice versa, particularly for memory B cells, suggesting possible low-level undetected parasitemia may have occurred in some individuals. The magnitude of MSP-142-specific memory B cells and levels of antibodies to MSP- 142 also did not differ from 2008 to 2009 (P.0.10 for both). However, from 2008 to 2009 the proportions of both classswitched atypical (CD19+IgD-CD27-CD21-IgM-) and class-switched activated (CD19+IgD-CD27+CD21-IgM-) memory B cells decreased (both P,0.001). In contrast, class-switched resting classical memory B cells (CD19+IgD-CD27+CD21+IgM-) increased (P,0.001). In this area of seasonal malaria transmission, a one- year absence of detectable P. falciparum infection was not associated with changes in the prevalence or level of MSP-142 specific memory B cells, but was associated with major changes in overall memory B cell subsets.
Maue, A (2024). Changes in b cell populations and Merozoite surface protein-1-specific memory b cell responses after prolonged absence of detectable p. falciparum infection. Afribary. Retrieved from https://track.afribary.com/works/changes-in-b-cell-populations-and-merozoite-surface-protein-1-specific-memory-b-cell-responses-after-prolonged-absence-of-detectable-p-falciparum-infection
Maue, Ayieko "Changes in b cell populations and Merozoite surface protein-1-specific memory b cell responses after prolonged absence of detectable p. falciparum infection" Afribary. Afribary, 16 Jul. 2024, https://track.afribary.com/works/changes-in-b-cell-populations-and-merozoite-surface-protein-1-specific-memory-b-cell-responses-after-prolonged-absence-of-detectable-p-falciparum-infection. Accessed 27 Nov. 2024.
Maue, Ayieko . "Changes in b cell populations and Merozoite surface protein-1-specific memory b cell responses after prolonged absence of detectable p. falciparum infection". Afribary, Afribary, 16 Jul. 2024. Web. 27 Nov. 2024. < https://track.afribary.com/works/changes-in-b-cell-populations-and-merozoite-surface-protein-1-specific-memory-b-cell-responses-after-prolonged-absence-of-detectable-p-falciparum-infection >.
Maue, Ayieko . "Changes in b cell populations and Merozoite surface protein-1-specific memory b cell responses after prolonged absence of detectable p. falciparum infection" Afribary (2024). Accessed November 27, 2024. https://track.afribary.com/works/changes-in-b-cell-populations-and-merozoite-surface-protein-1-specific-memory-b-cell-responses-after-prolonged-absence-of-detectable-p-falciparum-infection