ABSTRACT
In recent years, there has been an increase in fatal road accidents in Namibia claiming many lives each year. Traffic accidents are often related to the use of alcohol, illegal drugs or psychoactive medicines. A rapid low volume dispersive liquid-liquid microextraction (DLLME) method in combination with gas chromatography-mass spectrometry (GC-MS) detection was developed for the simultaneous determination of illicit psychoactive drugs in blood (those used most commonly in Namibia: cocaine, ∆⁹- tetrahydrocannabinol, amphetamine and methamphetamine). Various DLLME parameters were optimised to improve the extraction efficiency of the analytes. The method was successfully applied for the simultaneous determination of the drugs from spiked blood in a single extraction with satisfactory sensitivity, accuracy, repeatability, linearity and recoveries. The limits of detection (LOD) for different drugs varied from 0.00001 to 0.055 µg/mL while the limits of quantification (LOQ) varied from 0.0001 to 0.1 µg/mL. The precision was in a range of 2-10 %. The method was linear in the ranges of 0.00001-10 µg/mL with the correlation coefficient (r2 ) ranging between 0.998-0.999. The recoveries of the analytes were between 84 and 103 %. The validated method was subsequently successfully applied in the screening of drugs in the blood collected from motorists (samples which were originally collected for blood-alcohol determination). The developed method utilised very low volumes of dispersion and extraction solvents (about 6 and 4 µL respectively) and only 25 µL of blood.
SIMASIKU, B (2021). Development Of A Low Volume Dispersive Liquid-Liquid Microextraction Method For The Simultaneous Determination Of Illicit Psychoactive Drugs Used By Motorists In The Khomas Region Of Namibia. Afribary. Retrieved from https://track.afribary.com/works/development-of-a-low-volume-dispersive-liquid-liquid-microextraction-method-for-the-simultaneous-determination-of-illicit-psychoactive-drugs-used-by-motorists-in-the-khomas-region-of-namibia
SIMASIKU, BRONAH "Development Of A Low Volume Dispersive Liquid-Liquid Microextraction Method For The Simultaneous Determination Of Illicit Psychoactive Drugs Used By Motorists In The Khomas Region Of Namibia" Afribary. Afribary, 20 Apr. 2021, https://track.afribary.com/works/development-of-a-low-volume-dispersive-liquid-liquid-microextraction-method-for-the-simultaneous-determination-of-illicit-psychoactive-drugs-used-by-motorists-in-the-khomas-region-of-namibia. Accessed 27 Nov. 2024.
SIMASIKU, BRONAH . "Development Of A Low Volume Dispersive Liquid-Liquid Microextraction Method For The Simultaneous Determination Of Illicit Psychoactive Drugs Used By Motorists In The Khomas Region Of Namibia". Afribary, Afribary, 20 Apr. 2021. Web. 27 Nov. 2024. < https://track.afribary.com/works/development-of-a-low-volume-dispersive-liquid-liquid-microextraction-method-for-the-simultaneous-determination-of-illicit-psychoactive-drugs-used-by-motorists-in-the-khomas-region-of-namibia >.
SIMASIKU, BRONAH . "Development Of A Low Volume Dispersive Liquid-Liquid Microextraction Method For The Simultaneous Determination Of Illicit Psychoactive Drugs Used By Motorists In The Khomas Region Of Namibia" Afribary (2021). Accessed November 27, 2024. https://track.afribary.com/works/development-of-a-low-volume-dispersive-liquid-liquid-microextraction-method-for-the-simultaneous-determination-of-illicit-psychoactive-drugs-used-by-motorists-in-the-khomas-region-of-namibia