EFFECT OF DIETARY SUPPLEMENTATION OF EMILIA SONCHIFOLIA LEAVE ON GLOBULIN AND BILIRUBIN LEVEL ON WEANLING RAT

color:windowtext">ABSTRACT

The
place of adequate nutrition in the live of the young especially in the recently
weaned cannot be overemphasized. The abrupt change in diet, leading to possible
immune system weakness and disease conditions, is a focal point in the care of
the young. This project examined serum globulin and bilirubin levels in
weanling rats whose feed was supplemented with Emilia sonchifolia leaf extract. The extract was also screened for
phytochemical contents and the effect of its supplementation in liver enzymes (AST,
ALT and ALP) activities.

TABLE OF CONTENTS

Title page

Abstract

Dedication

Acknowledgement

Certification

Table of Contents

List of Tables

color:windowtext">CHAPTER ONE:         INTRODUCTION

1.1     Background
of the study                                                                   1

1.2     Statement
of the problem                                                        7

13      Aim
and Objectives of the Study                                             8       

1.4     Research
questions                                                                  9

1.5     Significance
of the study                                                                   10

1.6     Scope
of the study                                                                            10

color:windowtext">CHAPTER TWO:        LITERATURE
REVIEW

2.1     Emilia sonchifolia: History and Ethnobotanical
applications  11

2.2     Botanical
description                                                              14

2.3     Proximate
Composition of Emilia sonchifolia leaf                            17

2.4     Anti-nutritional
Factors                                                          18

 

2.5     Effect
of oral administration of Emilia sonchifolia leaf

 in Wistar rats                                                                          19

2.6     Serum
globulin and its clinical relevance                                 21

2.7     Serum
bilirubin and its biochemical effects in animal              23

2.8     Kidney
function parameters and its relevance in health

and disease                                                                              25

color:windowtext">CHAPTER THREE:    MATERIALS
AND METHODS

3.1     Materials                                                                                 27

3.2     Site
of Study                                                                           27

3.3     Plant
collection and extract preparation                                  27

3.4     Experimental
animals                                                              28

3.5     Experimental
diet                                                                    29

3.6     Metabolic
studies                                                                    29

3.7     Methods                                                                                  29

3.7.1  Estimation of serum globulin using (manufacturer protocol)           29

3.7.2  Estimation of serum bilirubin level                                         30

3.8     Quercetin
standard drug (administered as a single dose                   

          in
one group of experimental animals)                                    30

3.9     Economic
Analysis/Limitation                                                          31

3.10   Statistical Analysis                                                                  31

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color:windowtext">CHAPTER FOUR:       RESULT
AND DISCUSSION

4.1     Results

4.2     Discussion

color:windowtext">CHAPTER FIVE:        SUMMARY
AND CONCLUSION

5.1     Summary

5.2     Conclusion

5.3     Recommendations

          References