Establishing A Method For The Mass Propagation Of Piper Guineense (Schumach) Via Tissue Culture

ABSTRACT

Piper guineense (Schumach) has great potentials for economic exploration because of the

proven use of its medicinal content in human health. The plant material is usually

obtained from thick forests by women who have to walk long distances in the bush to

reach it. A technique that improves its propagation and domestication, such as tissue

culture, becomes necessary. This study was therefore initiated to develop a method for

the mass propagation of Piper guineense seedlings using in vitro regeneration. Plants

react differently to media concentrations and constituents for their in vitro regeneration.

Comparative growth of Piper guneensee inoculated on Murashige and Skoog(MS)

medium supplemented with some growth regulators were investigated. Mature nodes

were collected from the medicinal garden of National Centre for Genetic Resources and

Biotechnology (NACGRAB) Ibadan. Nodes of the species were inoculated onto MS

media. The MS media was supplemented with the following concentrations of growth

regulators 0.25, 0.50, 0.75, 1.0mg/l. BAP, the same concentration for KIN and 0.05mg/l

of NAA for all replicate. After two weeks of inoculation it was observed that, the plant let

with BAP 0.50mg/l +NAA 0.05mg/l were found contamination-free and sprouted, while

other treatments showed contamination including control. Furthermore, after 7 days the

sprouted, plantlets with treatment of BAP 0.50mg/l +NAA 0.05mg/l were also

contaminated, thereby yielding no positive result. Many repeats of the experiment also yielded contaminated products

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