Extraction of DNA in Tomatoes and Garden Eggs Fruits

ABSTRACT

This project is basically concerned with the extraction of DNA in tomatoes and garden egg fruit. An experiment on how to extract DNA on tomatoes and garden egg, isolation on it to see what DNA actually look like as well as importance of DNA extraction are consid3ered in this project.

TABLE OF CONTENT

Title page ……………………………………………………… i.

Certification……………………………………………………..ii

Dedication……………………………………………………….iii

Acknowledgement……………………………………………..iv

Abstract………………………………………………………….v

Table of content……………………………………………….vi

CHAPTER ONE

1.0      Introduction………………………………………………1

1.1      History of DNA…………………………………………..1-3

1.2      Definition of DNA……………………………………….6

1.3      Definition of extraction of DNA……………………….6

1.4      Importance of DNA extraction……………………7

1.5      The human Genome project………………………7-12

1.6      How genes are sequence……………………………..12

CHAPTER TWO

2.0      Literature review………………………………………13

2.1      Concept of DNA replication…………………………13

2.2      Eukaryotic DNA replication………………………13-17

2.3      Protein associated with DNA replication and phase keeping…………………………………………………18-23

CHAPTER THREE

3.0      Materials and methodology……………………………24

3.1      Collection of the samples……………………………..25

3.2      Preparation of the samples………………………25-26

3.3      Procedure………………………………………………26

3.3.1 Preliminary operations…………………………………27

3.3.2 Preparation of the extraction solution……………..27

3.3.3 Preparation of the Pulp………………………………..28

3.3.4 Filtration of the Pulp…………………………………...28

3.3.5 Extracting of DNA……………………………………….29

3.3.6 Extracting of DNA………………………………….29-34

CHAPTER FOUR

4.0      Result…………………………………………………35-36

CHAPTER FIVE

5.0      Discussion……………………………………………37-39

5.1      Conclusion……………………………………………..40

5.2      Recommendation………………………………………41

References……………………………………………42-44