A CMOS biosensor with a folded floating-gate is created to detect charged biochemical molecules. It contains a FET, a control-gate and a sensing area. The floating-gate spans the whole device, allowing the sensing area to be placed on top of the FET, resulting in a decrease of the device's total area. The device is sensitive to the polarity and quantity of charged poly amino acids and could be used for electronic recognition of temporal and spatial migration of charges, such as in biological ...
A CMOS biosensor with a folded floating-gate is created to detect charged biochemical molecules. It contains a FET, a control-gate and a sensing area. The floating-gate spans the whole device, allowing the sensing area to be placed on top of the FET, resulting in a decrease of the device's total area. The device is sensitive to the polarity and quantity of charged poly amino acids and could be used for electronic recognition of temporal and spatial migration of charges, such as in biological ...
The emergence of new drugs is often driven by the escalating resistance of parasites to existing drugs and the accessibility of more advanced technology platforms. Microfluidic devices have allowed for quicker testing of compounds, regulated sampling/sorting of entire animals, and automated behavioral pattern detection. In the majority of microfluidic devices, the effects of drugs on small animals (e.g. Caenorhabditis elegans)elegant are quantified by an endpoint, dose response curve that sho...
In this study, two sets of experiments were conducted in order to investigate the impact of static magnetic fields on the growth and ethanol production of Saccharomyces cerevisiae. The first experiment ran for 25 hours with a 2% dextrose loading rate, while the second ran for 30 hours with a 6% dextrose loading rate. The magnetic fields used were homogeneous and non-homogeneous, with strengths of 100 mT and 200 mT, respectively. The results showed that the homogenous magnetic field had no sig...
We have created a microfluidic system that is open and lets us dispense and control discrete droplets on flat plastic sheets. To do this, a superhydrophobic material is sprayed on pre-made plastic sheets and hydrophilic symbols are printed onto them with an inkjet printer. The patterned plastic sheets are connected to a two-axle tilting platform, which is powered by stepper motors, and gives the droplets a mechanical movement. We have tested multiple droplet operations, such as; delivery of d...
Multiphase slug flow is going to be analysed while focusing on how pressure is lost and monitoring the velocity profiles. The problem involved in a multiphase flow is by far more complex than that which could be observed in a single phase flow. The fluid coming from the wellbore and onto the first part of the separator is conventionally a two phase flow of liquid/gas (air and silicone oil in this study). Using large diameter pipe and industrial based fluids, focus would be laid on flow direct...
Apparatus , methods , and systems for automated liquid drop let manipulation include an open droplet supporting surface. An actuator can translate the surface in space with at least one degree freedom of movement to influence movement of one or more droplets on the surface . In one embodiment , the surface is patterned with areas that attract the droplets and interstitial areas that repel the droplets to enhance transport of droplets . For example , for water - based droplets the attr...
In recent years, there has been significant interest in developing wearable devices to mimic the integrated sensing of life forms, which enhances their performance and survival capabilities. Progress in the development of physical sensors and wearable electronics has been promising, leading to numerous consumer products that measure activity, posture, heart rate, respiration rate, and blood oxygen level. Despite the challenges in retrieving and processing bodily fluids, wearable chemical sens...
In recent years, there has been significant interest in developing wearable devices to mimic the integrated sensing of life forms, which enhances their performance and survival capabilities. Progress in the development of physical sensors and wearable electronics has been promising, leading to numerous consumer products that measure activity, posture, heart rate, respiration rate, and blood oxygen level. Despite the challenges in retrieving and processing bodily fluids, wearable chemical sens...
This study uses the model organism, C. elegans, to investigate its sensitivity and response to static magnetic fields. Wild-type C. elegans are put into microfluidic channels and exposed to permanent magnets for five cycles of thirty-second time intervals at field strengths ranging from 5 milli Tesla to 120 milli Tesla. Recorded and analyzed with custom software, the results of the worm's movement - the average velocity, turning and curling percentage - were compared to control experiments...
To evaluate the level of infestation of the soybean cyst nematode (SCN), Heterodera glycines, in the field, egg population densities are determined from soil samples. Sucrose centrifugation is a common technique for separating debris from the extracted SCN eggs. We have developed a procedure, however, that employs OptiPrep as a density gradient medium, with improved extraction and recovery of the eggs compared to the sucrose centrifugation technique. Also, we have built computerized methods t...
Soybeans are an important crop for global food security. Every year, soybean yields are reduced by numerous soybean diseases, particularly the soybean cyst nematode (SCN). It is difficult to visually identify the presence of SCN in the field, let alone its population densities or numbers, as there are no obvious aboveground disease symptoms. The only definitive way to assess SCN population densities is to directly extract the SCN cysts from soil and then extract the eggs from cysts and count ...
Previous studies have produced mixed results on the influence of magnetic fields on the fermentation kinetics and growth of various types of Saccharomyces cerevisiae. We carried out two experiments to assess the role of magnetic fields on cell growth and ethanol production during fermentation. The first of these experiments took place for 25 h, with a dextrose loading rate of 2%, and the magnetic fields were both homogeneous and non-homogeneous with intensities of 100 and 200 mT. The second e...
A method to create smart and flexible switches for the regulation of liquid flow across multiple channels is essential in paper microfluidics. Prior approaches are hampered by long response times, high actuation fluid volumes, and external control circuitry. To diminish these problems, we designed a distinctive actuator device fashioned entirely from chromatography paper and featuring folds. The fold can be selectively wetted by an actuation fluid at either the crest or trough, resulting in t...
A CMOS biosensor with a folded floating-gate is created for the detection of charged biochemical molecules. It is made up of a field-effect transistor with a floating-gate, a control-gate, and a sensing area. The molecules on the sensing area result in a voltage being induced on the floating-gate and a shift in the threshold characteristics. The floating-gate spans the entire device, which allows for the sensing area to be placed above the FET. This reduces the total device area. The device d...