Environmental & Physical Sciences

Environmental & Physical Sciences Research Papers/Topics

Infestation Levels and Molecular Identification Based on Mitochondrial COI Bar code Region of Five Invasive Gelechiidae Pest Species in Kenya

Abstract Invasive Gelechiidae pest species, namely Tuta absoluta, Phthorimaea operculella, Aproaerema simplixella, Sitotroga cerealella, and Pectinophora gossypiella are among the major constraints hampering agricultural economy in Kenya. Infestation levels were determined on respective host crops sampled from different localities and P. operculella recorded the highest infestation of 68.00 ± 4.92% on stored potato. Aproaerema simplixella and T. absoluta accounted for 61.33 ± 5.35% and 51....

Common Cerambycid Pheromone Components as Attractants for Longhorn Beetles (Cerambycidae) Breeding in Ephemeral Oak Substrates in Northern Europe

Abstract Longhorn beetles are ecologically important insects in forest ecosystems as decomposers of woody substrates, microhabitat engineers, and as components of forest food webs. These species can be greatly affected both positively and negatively by modern forestry management practices, and should be monitored accordingly. Through headspace sampling, coupled gas chromatographyelectroantennography, gas chromatography-mass spectrometry, and field bioassays, we identified two compounds, 2-me...

Plant parasitic nematode assemblages associated with sweet potato in Kenya and their relationship with environmental variables

Abstract Sweet potato is one of the most important staple food crops consumed in Kenya and throughout Africa but yields are greatly reduced by plant parasitic nematodes (PPN). The aim of this study was to determine the prevalence of PPN in Kenyan sweet potato fields and their relationship with soil and climatic variables. Soil samples were collected from sweet potato fields in Busia, Teso, Kisii, Embu and Makueni counties. Thirteen nematode genera were identified across the five counties wit...

Diversity of fungi in sediments and water sampled from the hot springs of Lake Magadi and Little Magadi in Kenya

Abstract Lake Magadi and Little Magadi are saline, alkaline lakes lying in the southern part of Kenyan Rift Valley. Their solutes are supplied by a series of alkaline hot springs with temperatures as high as 86°C. Previous culture-dependent and independent studies have revealed diverse prokaryotic groups adapted to these conditions. However, very few studies have examined the diversity of fungi in these soda lakes. In this study, amplicons of Internal Transcribed Spacer (ITS) region on Tota...

Disappearing medicinal plants in mt. kenya forests, kenya: a case study of east african green heart (Warburgia ugandensis sprague)

Abstract Natural disturbances and human activities are significantly affecting indigenous trees species in forests. Warburgia ugandensis is a plant highly valued for its medicinal properties, timber and fuel among other uses. Many rural communities use it as a remedy for a number of ailments. Investigations into the value of its chemical content reveal that it has antibiotic, antifungal, anti inflammatory and antiseptic effects. W. ugandensis has been rated as the second highest medicinal pl...

Toxic cyanobacteria and their toxins in standing waters of Kenya: implications for water resource use

Abstract Phytoplankton biodiversity studies in Kenya's standing waters were carried out between 2001 and 2003. Toxin producing cyanobacteria were recorded in twelve water bodies. Microcystis and Anabaena were the most common species in freshwaters while Anabaena and Anabaenopsis were common in alkaline saline lakes. Seven lakes with cyanobacteria blooms and a hot spring had detectable levels of microcystins and anatoxin-a. Cell bound microcystins (LR equivalents) concentration ranged from 1....

Changes of phytoplankton communities in Lakes Naivasha and Oloidien, examples of degradation and salinization of lakes in the Kenyan Rift Valley

Abstract Increasing degradation of the water quality, caused by overuse and salinization, leads to considerable changes of the phytoplankton composition in Kenyan Rift Valley lakes. Exemplarily, the phytoplankton communities and biomasses of deteriorating freshwater Lake Naivasha and salinizing Lake Oloidien were studied between 2001 and 2005, accompanied by physico-chemical measurements (pH, total phosphorus and nitrogen, alkalinity, conductivity). Over the last three decades, the ecology o...

Genetic diversity among commercial coffee varieties, advanced selections and museum collections in Kenya using molecular markers

Abstract Molecular markers have effectively been applied to study genetic diversity and as markers of particular traits. This study assessed the diversity of twenty four (24) coffee genotypes using 10 Random Amplified Polymorphic DNA (RAPD) primers and 2 microsatellites (M24 and Sat 235). A total of 35 polymorphic bands were generated by the RAPD primers. The bands were scored for presence (1) and absence (0) of amplified products. The data was subjected to cluster analysis using R statistic...

Isolation, characterization and identification of Diazinon degrading bacteria from the soil and gut of macrotermes

Abstract Diazinon degrading bacteria were isolated from the soil and gut of fungus cultivating termites Macrotermes michaelseni using MM7 media supplemented with pesticide as the sole source of carbon and energy. The isolates were gram negative rods the isolates from the soil were designated DS2, DS3 and DS5 while from termite gut was designated DT2. 16S rRNA gene sequence analysis showed that diazinon degrading isolate DS2 (from the soil) is closely related to Achromobacter xylosoxidan AF50...

Chemical properties associated with guts, soil and nest materials of Odontotermes and Macrotermes species from Kenya

Abstract Changes in carbon and nitrogen cycles in tropical soils affect soil functioning and ecosystem activity. Termites play important roles in carbon and nitrogen cycles, thus determination of levels of such compounds is essential. This study was aimed at determining the levels of various forms of nitrogen, carbon and pH in the guts, soil and nest materials associated with Odontotermes and Macrotermes termite species. Macro- and micro-elements such as potassium, calcium, magnesium, phosph...

Mitochondrial DNA variation of Bemisia tabaci (gennadius) (hemiptera: aleyrodidae) infesting cassava in Kenya

Abstract Bemisia tabaci is a widely distributed crop pest affecting the yield of a broad range of agricultural, fiber, vegetableand ornamental crops. It is an extremely polyphagous pest that causes direct damage and can act as a vector ofviral plant diseases. Populations of Bemisia tabaci that are morphologically indistinguishable and with differentbiological traits have been known to exist; they show differences in rates of development, host range, insecticideresistance and virus transmissi...

Effects of pea aphid secondary endosymbionts on aphid resistance and development of the aphid parasitoid Aphidius ervi: a correlative study

Abstract In order to reduce parasite-induced mortality, hosts may be involved in mutualistic interactions in which the partner contributes to resistance against the parasite. The pea aphid, Acyrthosiphon pisum Harris (Hemiptera: Aphididae), harbours secondary bacterial endosymbionts, some of which have been reported to confer resistance against aphid parasitoids. Although this resistance often results in death of the developing parasitoid larvae, some parasitoid individuals succeed in develo...

Infestation Levels and Molecular Identifcation Based on Mitochondrial COI Barcode Region of Five Invasive Gelechiidae Pest Species in Kenya

Abstract Invasive Gelechiidae pest species, namely Tuta absoluta, Phthorimaea operculella, Aproaerema simplixella, Sitotroga cerealella, and Pectinophora gossypiella are among the major constraints hampering agricultural economy in Kenya. Infestation levels were determined on respective host crops sampled from different localities and P. operculella recorded the highest infestation of 68.00 ± 4.92% on stored potato. Aproaerema simplixella and T. absoluta accounted for 61.33 ± 5.35% and 51....

Variation in Seagrass Carbon Stocks Between Tropical Estuarine and Marine Mangrove-Fringed Creeks

Abstract Seagrass and associated blue carbon ecosystems are important carbon sinks, and hence understanding their spatial and temporal variability is vital in appreciating their potential roles in climate change mitigation and adaptation. The Indo-Pacific region has the highest seagrass biodiversity, yet little focus has been made to compare seagrass habitat extent and carbon dynamics with their temperate counterparts. The present study assessed habitat characteristics and seagrass species ...

Seagrass Loss and Sub-Surface Carbon Fate: Insights from a Long-Term Experimental Removal in Gazi Bay, Kenya

Abstract Seagrass meadows are considered as global hotspots of blue carbon stocks. However, they suffer global cover loss mainly due to anthropogenic activities. Few is known on the impact of seagrass loss on their blue carbon stocks. This study investigates the impact of seagrass removal on soil organic carbon stocks two years after initial perturbation, and the potential bioturbation activity of co-existing burrowing shrimps in Gazi Bay, Kenya. Seagrass aboveground biomass was removed for ...


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