Biotechnology Research Papers/Topics

EFFECT OF BIOCHAR AMENDMENT RATES AND BIO- NEMATICIDE ON GROWTH, YIELD AND ROOT-KNOT NEMATODES (MELOIDOGYNE SPP.) INFESTATION OF TOMATO (SOLANUM LYCOPERSICUM L.)

Pot and field experiments were conducted at the Nyankpala Campus of University for Development Studies from January 2020 to March 2022. The objective was to test the efficacy of peptide-based nematicide (Nemanol) and its concentration on root-knot nematodes and growth and yield of tomato in biochar amended soil. In experiment 1, the treatments which were applied in a completely randomised design (CRD) with three replications were; Soil with no biochar and no nematicide (T1), Soil with 5 ml of...

EFFECT OF TEMPERATURE PRE-TREATMENT ON BIOGAS PRODUCTION FROM Calotropis procera (Aiton) LEAVES AND COW DUNG UNDER ANAEROBIC CONDITION

Abstract: Biogas is an alternative and renewable energy source produced through anaerobic digestion of organic matter. Low degradability of substrates is one of the factors that obstruct the production of biogas. With the aim of evaluating biogas yields from Calotropis procera (CP) leaves and cow dung (CD) a sequence of experiments were carried out under mesophilic conditions at 38ºC using batch digester operating for 21 days. The pre-treatments of biogas were done at 60 and 80ºC temperatu...

BIOGAS PRODUCTION FROM CO-DIGESTION OF COW MANURE AND AVOCADO FRUIT PEEL WASTE

Abstract: Energy is one of the most important factors for human development and to global economic growth. Biogas is a mixture of gases, mainly methane and carbon dioxide, resulting from anaerobic digestion of organic matter. In this study, biogas production from co-digestion of cow manure (CM) and avocado fruit peel (AF) waste in five mix ratios (100%CM, 60%CM:40%AF, 50%CM:50%AF, 40%CM:60%AF and 100%AF) were evaluated under mesophilic condition at 38ºC using a batch mode digester in Microb...

PRODUCTION OF BIOGAS FROM Desmodium triflorum (L.) THROUGH ANAEROBIC CO-DIGESTION WITH COW DUNG

Abstract: Biogas is a methane rich gas produced by anaerobic breakdown of organic wastes with the help of methanogenic Archaebacteria under oxygen free environment. The objective of this study was to evaluate the potential of Desmodium triflorum (DT) to produce biogas through anaerobic digestion alone or in combination with cow dung (CD). In this study, biogas production from co-digestion of the Desmodium triflorum with cow dung in five mix ratios was evaluated under mesophilic conditions (3...

ANAEROBIC DIGESTION OF PARTHENIUM WEED WITH GOAT MANURE TO GENERATE BIOGAS ENERGY

Abstract: Biogas is an alternative and renewable energy source produced through anaerobic digestion of organic matter. The aim of this study was to obtain the optimal mix ratio for biogas production from anaerobic digestion of Parthenium weed and goat manure. The experiment was batch operated and daily gas yield from the plant was monitored for 30 days. The Parameters studied were total solids (TS), volatile solids (VS), fixed solids, organic carbon, and moisture content. For the experimenta...

IN VITRO PROPAGATION OF BANANA (Musa paradisiaca L.) PLANT USING SHOOT TIP EXPLANT

Abstract: Bananas are multipurpose fruit crops which has great socio-economic importance in the world and there is a very high demand of disease free banana planting material by small and large scale farmers in Ethiopia. However, Conventional propagation methods are in general inefficient due to their low propagation rate, time consuming and disease dissemination. Thus, the objective of this experiment was to develop a proper and reproducible protocol for in vitro propagation of banana cv. G...

IN VITRO REGENERATION OF CHENIN BLANC AND CANONANNON VARIETIES OF GRAPEVINE (Vitis vinifera L.) FROM LEAF EXPLANTS

Abstract: Grapevine (Vitis vinifera L.) is one of the most widely distributed fruit crops in the world. The availability of adequate amount of quality and disease free planting materials within a short time is the major limiting factor to attain large scale grapevine production using the conventional method of Propagation. Therefore, development of an optimal in vitro propagation technique is too much needed. The objective of this study was to develop an optimal in vitro regeneration protoco...

EFFECT OF TEMPERATURE PRE-TREATMENT ON BIOGAS PRODUCTION FROM BARLEY STRAW AND GOAT MANURE UNDER ANAEROBIC CONDITION

Abstract: Biogas technology, which is meant to produce methane rich gas through anaerobic digestion of biological materials, is an environmentally friendly technology that reduces emission of green house gases. Therefore, this research was initiated to utilize biomasses such as barley straw (BS) and goat manure (GM) for biogas production. In this study, biogas production from co-digestion of the BS and GM in five mix ratios (100%BS, 75%BS: 25%GM, 50%BS: 50%GM, 25%BS: 75%GM and 100%GM mix) we...

BIO-ORGANIC LIQUID FERTILIZER PRODUCTION FROM CHICKEN MANURE AND BANANA PEELS AND EVALUATING ITS EFFECTIVENESS ON LETTUCE (Lactuca sativa L.) UNDER HYDROPONIC CONDITION.

Abstract: Bioorganic liquid fertilizers were produced from locally available substrates like chicken manure and banana peels. It was obtained through aerobic fermentation comprises a number of growth substances, vitamins, antibiotics, amino acids and useful micro-organisms. Bioorganic liquid fertilizer increases fertility of crops and also accelerates plant maturation through improved nutrient quality. Thus, the present study was aimed to produce bioorganic liquid fertilizer from chicken man...

PRODUCTION OF BIOGAS FROM Cucurbita pepo L. LEAVES CODIGESTED WITH COW DUNG

Abstract: Biogas is eco-friendly alternative renewable energy source produced through anaerobic digestion of organic compounds. In this study, biogas production was evaluated from Cucurbita pepo leaves (CP) and cow dung (CD) co-digestion in five mix ratios under mesophilic conditions (38ºC) using batch digesters in the Botany Laboratory of Haramaya University. Total solid(TS) and Volatile solid(VS), organic carbon, and pH were measured before and after digestion, While carbon to nitrogen ra...

HYDROPONIC GROWING OF LETTUCE (Lactuca sativa L.) AND PRODUCTION OF BIOORGANIC LIQUID FERTILIZER FROM CHICKEN MANURE AND ONION PEELS.

Abstract: Lettuce (Lactuca sativa L.) is one of the most popular leafy salad vegetables for fresh consumption and containhealth-promoting compounds.Hydroponic method is a techniques of cultivation plants using bioorganic liquid fertilizer without soil and is more important than soil because of efficient resourcesmanagement and quality food production. This study,wasaimed to produce bioorganic liquid fertilizer from fermented of fresh chicken manure and onion peels that can be used for hydrop...

Biogas Production from Wheat Straw Co-Digested With Poultry and Goat Manure

Abstract: Biogas production using anaerobic digestion of organic waste materials provides an alternative environmentally friendly renewable energy. In this study, the production of biogas from co-digestion of wheat straw with poultry and goat manure was evaluated in seven treatment under mesophilic condition 380C for30 days of retention time, using a batch digester in Botanical and Central Laboratories of Haramaya University. Total solids, volatile solids, percent moisture content, pH, organ...

IN VITRO PROPAGATION OF SELECTED SUGARCANE (Saccharum officinarum L.) VARIETIES (C 86-12 and C 86-165) THROUGH APICAL MERISTEM

Abstract: Sugarcane (Saccharum officinarum L.) is monocotyledonous crop plant that belongs to the Poaceae family that mostly propagates through conventional methods. However, conventional propagation lacks rapid multiplication procedures to commercialize newly released varieties within a short period of time. Hence, the objective of this work was to optimize in vitro micro propagation protocol for two sugarcane varieties (C 86-165 and C 86-12) using shoot apical meristem explants. The two va...

PRODUCTION OF BIOGAS FROM AN INVASIVE WEED BIOMASS (Argemone ochroleuca L.) CO-DIGESTED WITH COW MANURE UNDER ANAEROBIC CONDITON

Abstract: Biogas is an alternative and renewable energy source produced through anaerobic digestion of organic matter whereby the organic matter is converted into a combustible gas rich in methane (CH4),solid residue(sludge) and a liquid effluent. The aim of this study was production of biogas from Argemone ochroleuca co-digested with cow manure under mesophilic conditions (38ºC) using batch digester for 30 days of fermentation time. The experimental design was completely random design with...

RODUCTION AND PARTIAL CHARACTERIZATION OF LACCASE FROM OYSTER MUSHROOM (Pleurotus ostreatus) COLLECTED FROM THE BARK OF EUCALYPTUS TREE

Abstract: The aim of the current study was to evaluate the effect of different growth substrates on laccase production and to partially characterize it in terms of the effect that pH, temperature and substrate concentration had on its activity. The study was conducted at the National Agricultural Biotechnology Research Center, Holetta. The fruiting bodies of Pleurotus ostreatus were collected from live old Eucalyptus tree barks. The potential of Pleurotus ostreatus in laccase production was ...


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