Haemopoietic And Haematinic Potentials Of Crude Methanolic And Aqueous Seed Extract Of Telfairia Occidentalis In Albino Wistar Rats.

ABSTRACT

Haemopoietic and Heamatinic potentials of crude methanol and aqueous seed extract of Telfairia

Occidentalis (Ugu) in albino rats were studied using one hundred and twenty eight rats of mixed sexes

ages 2-3 month weighing (150+ 20)g. Acute toxicity studies was performed and LD50 of the Telfairia

Occidentalis obtained using standard methods. This work was carried out in the animal house of the

University of Nigeria, Enugu Campus. The work was done in two set. The first set, the aqueous seed

extract, the rats were divided into nine groups of eight animals each Group A, B1-B3, C-G. Group A

was the normal control. Group G was the negative control; Group F was the positive control. Groups

A, B1-B3 were used for haemopoietic potential while groups C-G were used for haematinic potential

and were first induced for anaemia using phenylhydrazine for 9 days. At the end of the induction, the

animals were given graded extract concentration, Group B1 – B3 received 50, 100 and 200mg/kg

body weight respectively also Groups C-E respectively received 50,100 and 200mg/kg body weight of

extracts. Group A, the normal control received only feed and water. Group F, the positive control

received 100mg/kg body weight of Astymin(a commercially prepared haematinic) while Groups G,

the negative control received only feed and water after induction of anaemia. The bloods was

collected from the animals on interval of 3days (day 3, day 6 and 9) from the onset of the

administration of the extracts and were distributed appropriately into di-potassium EDTA for

haematology studies and plain bottles for liver enzymes. The rats were sacrificed after collection of

blood, the bone marrow was collected, the liver and kidney extracted for histological studies. The

second set was used for the methanol extract, here the seeds were extracted in methanol and the

process repeated as in the case of aqueous extract. Results obtained showed that the reticulocytes of

the rats that received the various concentration of both methanol and aqueous extract were

significantly increased p