In Vitro Antibacterial Activity Of Acacia Nilotica (Algarad) Methanolic And Aqueous Extracts Against Wound Pathogens

Aimed to this study was to study the antibacterial activity of the different concentrations of methanolic and aqueous extract of the medicinal plants A.nilotica sub spp nilotica and sub spp adansonii pods using agar dilution method. Hundred wound swabs were collected from patients with infected wounds who attended to Soba Teaching Hospital and Alban Gadeed Teaching Hospital during period from March to October 2015. From this study it was found that wound infection had high frequency in patients of age 21-40 years. S.aureus was major isolates, and no significant association was found between wound infection and history of disease (P value 0.872). The methanol and aqueous extract of A.nilotica pods (Family-Fabaceae) were screened for their antimicrobial activity against Standard bacteria; One Gram positive (S.aureus, ATCC25923) and Four Gram negative (K.pneumonae ATCC53657, P.aeurginosa ATCC 27853, E.coli ATCC 25922 and Proteus spp ATCC 6380). These extracts were also screened against clinical isolates of S.aureus, MRSA, E.coli, K.pneumonae, P.areuginosa, and Proteus spp. Methanol extract of A.nilotica sub spp nilotica and adansonii were more active against one or more of six bacteria. The aqueous extract pods of A.nilotica exhibited high activity against S.aureus. The antibacterial activity of 4 reference drugs: Gentamicin, Tetracycline, Ampicillin and Erythromycin were determined against the Standard bacteria and their activities were compared to the activity of plants extract. Gas Chromatography analysis exhibited 33 Compounds of A.nilotica sub and 15 compounds for sub adansonii, this different in the component may due to the environmental difference, also these compounds were identified and quantified by the Retention time and quantitatively by the area under the curve.