ISOLATION AND CHARACTERISATION OF ORAL MICROBES IN PERIODONTAL DISEASED PATIENTS ATTENDING FEDERAL COLLEGE OF DENTAL TECHNOLOGY AND THERAPY DENTAL

ABSTRACT

This study was conducted to isolate and characterize the oral microbes found in periodontal disease patients at dental clinic of Federal College of Dental Technology and Therapy, Enugu between March to October 2017. The tests carried out was done using  blood agar, Mac conkey agar and gram staining technique in which the result showed that organisms responsible for periodontal disease are majorly gram negative anaerobes which includes the Porphyromonas gingivalis, Fusobacterium, Prevotella intermedia and Tanerella forsythia. The gram positive organisms include the Streptococcus species, Lactobacillus and Actinomyces naeslundii. It was therefore recommended that oral hygiene practice must be emphasized which include mechanical brushing of teeth and regular scaling and polishing. 

TABLE OF CONTENTS
Cover page - - - - - - -
Table of Contents - - - - - - -
Abstract - - - - - - -
CHAPTER ONE- INTRODUCTION
Background of the Study - - - - - - 1
Statement of the Problem - - - - - - 3
Objective of the Study - - - - - - 4
Research Questions - - - - - - 5
Significance of the Study - - - - - - 5
CHAPTER TWO – LITERATURE REVIEW
Description of Periodontal Disease - - - - - 7
Normal Flora (Bacteria) of the Oral Cavity - - - - 11
The Oral Microbiota and Periodontal Disease - - - 14
Commensals in the Oral Cavity - - - - - 16
Biofilms and Oral Microbiota - - - - - 17
Associated Systemic Diseases Linked to Periodontal Disease - 19
Clinical Assessment of Periodontal Risk Factors - - - 24
Controllable and Uncontrollable Risk Factors - - - 25
Treatment of Periodontitis - - - - - - 32
CHAPTER THREE- RESEARCH METHODOLOGY
Materials and Methods - - - - - - - 38
Research Methodology - - - - - - - 38
Research Design - - - - - - - 38
Description of the Study Area - - - - - 38
Apparatus and Materials - - - - - - 38
Chemicals/ Reagents - - - - - - 40
Methods of Data Collection - - - - - - 41
Media Preparation - - - - - - - 41
Innoculation of Samples - - - - - - 42
CHAPTER FOUR- RESULTS
Colony of the Organism - - - - - - - 47
Motility Test - - - - - - - 49
Microscopic Morphology and Staining Reaction - - - 49
CHAPTER FIVE- DISCUSSION, CONCLUSION AND
RECOMMENDATIONS
Discussion - - - - - - - 51
Summary - - - - - - - 52
Conclusion - - - - - - - 53
Recommendation - - - - - - - 53
Definition of Terms - - - - - - - 55
References - - - - - - - 57
Appendix I: Images of Periodontal diseased teeth - - - 60
Appendix II: Images of Gram stained microbes - - - 61