Isolation And Identification Of Microorganisms Associated With Grounded Palm Kernel Shells Using Chicken Droppings As Innoculum At . Ratio 2:1.

ABSTRACT

This research study is designed to determine the microbial count of Chicken Droppings and Ground Palm kernel shell in which the chicken droppings is used as inoculum on Ground Palm Kernel Shell at Ratio 2: l in which the inoculum size is half of the Ground Palm Kernel Shell. Pollution of the environment due to oil production can be reduced through this study because is aimed at examining the microorganism responsible for the biodegradation that can be achieved in Palm kernel shell pollution after supplementing with organic manure( chicken droppings) .On every 5days ofbiodegradation 5g of the sample was picked and kept for microbial assay. The study demonstrated that chicken droppings contain more microorganisms that can breaks down quite rapidly. Bacteriological analysis was carried out on the samples for Isolation , characterization and identification of microorganisms associated with the biodegradation process as well as total aerobic plate count were carried out and compared over time with respect to the different concentrations. Biochemical test results on the isolates indicate that Palm kernel shell contained a variety of microorganisms which include Klebsiella pneumoniae ,Bacillus sp, Proteus sp, Pseudomonas, Staphylococcus aureus and Rhizopus sp. Analysis of Ground palm kernel shell mixed with chicken droP,pings revealed that the bacterial counts were encouraged while the proliferation of palm kernel shell degrading bacteria in the soil was depressed. The microorganism isolated are rod and coccibut rod are the dominating microorganism and most of the organism identified are gram(+) and gram(-) bacteria. The pH reading shows that Ground Palm Kernel inoculated with chicken droppings at day O to day 5 ranges from 4.59 pH - 6.02pH which is in acidic condition but from day I 0- day 30 the alkalinity increases which indicate that the Degrading microorganism are in alkaline condition. From Day 0-Day 30 the pH ranges from 4.59pH - 12.50pH. The Total Titratable Acid (TT A) shows that the degrading microorganism are able to produce organic acid in which the acidity increases over time. The count of the bacteria from Uncrushed Palm Kernel Shell ranged from 1.8 x 106(cfu/g) to 2.2 x 106 (cfu/g), while that of soil ranged from 7.2 x107 to 2.03 xl0 7 (cfu/g), the bacteria of the bacteria of chicken droppings ranged from 0.6 x 107 to 4.0 x 106 (cfu/g)' The degrading bacteria of palm kernel shell inoculated with chicken droppings from Day 0- Day 30 ranged from 1.96 x 107 to 0.5 x 107(cfulg) which indicate relatively high counts of bacteria and fungi in the sample