Isolation and Sensitivity of Bacteria Isolate from Vaginal Discharge to Antibiotics

ABSTRACT

• Normal vaginal flora contains a wide range of microorganisms. Bacterial vaginosis BV is the main reason for vaginal discharge. Many gram-positive and gram-negative rods i.e. E.coli, Klebsiella, Proteus, Acinetobacter and Pseudomonas spp. are major contributors to bacterial vaginosis. Aim: The present study was conducted to elucidate the frequency of various bacteria in high vaginal swabs and the sensitivity pattern of bacteria to antibiotics that are currently used Material and Methods used are a  total of 6 High vaginal swabs (HVS) which were collected from patients presenting with symptoms of vaginal discharge. Swabs were inoculated on blood and chocolate agar. After overnight incubation plates were examined for growth, and colonial morphology, and final confirmation was done on the basis of biochemical testing. Antibiotic sensitivity testing was done by (modified Kirby-Bauer’s) disc diffusion method using amikacin(30μg), ampicillin(10μg), amoxicillin(10μg),) clavulanic acid, imipenem(10μg),  ciprofloxacin(10μg),  and cefixime(5μg). After overnight incubation plates were examined to read the susceptibility zone. Results showed  that Highly sensitive antibiotics against bacteria were imipenem (27mm), and Ciprofloxacin (28mm)  whereas least affective antibiotics against gram negative rods were penicillins, amikacin due to indiscriminate use of antibiotics. In conclusion, high prevalence of gynecological infections demands that the patients who have vaginosis must be investigated regularly and carefully through culture and identification of causative bacteria. Emergence of antibiotic resistance must be controlled in order to avoid improper use, frequent abuse, insufficient dosages, trouble-free availability of antibiotics and treatment schedule must be designed subsequent to proper laboratory investigations.