STUDIES ON THE HAEMATOLOGICAL CHARACTERISTICS OF CLARIAS GARIEPINUS FROM GUBI DAM, BAUCHI STATE, NIGERIA

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ABSTRACTStudies were carried out to determine the haematological characteristics of Clarias gariepinus healthy specimens were obtained from Gubi dam. Blood sample ranging between 1.0-1.5ml were taken with the aid of sterilized syringe (21G) inserted by caudal vein puncture. The haematological parameters for each blood specimen were measured using automated haematological analyzer (sysmex Kx 21N; 000758 model). Blood biochemical analysis to determine plasma albumin, glucose, protein, sodium, potassium, inorganic phosphorus, were carried out using calometric and spectrophotometric methods. Results obtained reveal that haematologin (HB) for C.gariepinus. Similarly blood biochemical parameters analyzed and corresponding mean values suggest that albumin, protein, urea, glucose, sodium and potassium differed significantly. These findings suggest that haematological characteristics of C.gariepinus measured under stress-free condition could provide a reference standard and baseline in the assessment of physiological response of fish environmental stress and disease condition.

TABLE OF CONTENTS

CONTENTS PAGE

Cover page.....i

Title page.....ii

Declaration.....iii

Certification.....iv

Dedication..... v

Acknowledgement...vi

Table of content... .viii

Abstract......xi

CHAPTER ONE

1.0Introduction....1

1.1Statement of problem..2

1.2Justification....3

1.3Aims and objectives..4

CHAPTER TWO

2.0LITERATURE REVIEW..4

2.1The significance of Haematological parameter. 4

2.2Red Blood Cells (Erythrocytes)....... . 6

2.3White Blood Cells (Leucocytes)....... 7

2.3.1Lymphocytes.. 7

2.3.2Thrombocytes.. 7

2.3.3Monocytes... 8

2.4 Haemoglobin...9

2.6 Blood Biochemistry..9

2.6.1Albumin... 10

2.6.2proteins....12

2.7Biology and Ecology of Clarias gariepinus.....14

CHAPTER THREE

3.0MATERIALS AND METHOD......16

3.1Description of study site.......16

3.2Sample collection.. 16

3.2 Fish samples... 16

3.3Haematological Investigation.......16

3.3.1Blood sampling........16

3.3.2Haematological Estimation.......17

3.3.3Erythrocyte and Leucocyte count......17

3.3.4Haematocrit Estimation.......18

3.4Biochemical Analysis........18

CHAPTER FOUR

4.0RESULTS.........19

4.1Haematologial parameters of Clarias gariepinus....19

4.2Blood Biochemical parameters of Clarias gariepinus...21

4.3Blood plasma Electrolyte concentration of Claris gariepinus... . . . . . 21

CHAPTER FIVE

5.0DISCUSSION...22

5.1Comparative assessment of haematological characteristics of Clarias gariepinus. . . 22

5.2Comparative evaluation of Blood parameters..... 22

5.3Recommendation.. 23

5.4Conclusion.... 23

REFERENCES... .24

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STUDIES ON THE HAEMATOLOGICAL CHARACTERISTICS OF CLARIAS GARIEPINUS FROM GUBI DAM, BAUCHI STATE, NIGERIA

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