Microbiology Research Papers/Topics

Antibacterial and phytochemical properties of five chewing sticks

The antimicrobial activities of the ethanolic, Aqueous and Ethyl acetate extracts of five Nigerian medicinal plant used as chewing sticks were tested against five microorganism, three bacteria and two fungi. The five medicinal plant tested are Vitellaria paradoxa, Vitex doniana, Sarcorcephalus latiolia, Vernonia amygdalina, Olax subcorpidica and the microorganisns are Staphylococcus aureus, Neisseria sieca, Streptococcus mutans and Lactobacillus salivarius and Actinomycetes viscosus. Out of t...

The Study of Antimicrobial Activity of Partially Purified Ethyl acetate Extracts of Bridelia ferruginea on Clinical Isolates

Bridelia ferruginea is commonly grown in Western Tropical Africa. The purpose of this research work is to determine the antimicrobial activities, qualitative and quantitative phytochemical screening, elemental composition, proximate analysis and anti-nutrient composition of purified fraction of Bridelia ferruginea extracts. The organisms used for this research are Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, Klebsiella pneumonia ATCC 35659, Pseudomonas aeruginosaATCC 25619,a...

BIODIVERSITY CONSERVATION AND ECOSYSTEM SERVICES FOR NATIONAL DEVELOPMENT

ABSTRACT  The most amazing feature of Earth is the existence of life, and the most extraordinary feature of life is its diversity. Human beings can coexist only with the abundance of and diversity of nature. It is widely acknowledged that biodiversity underpins ecosystem functioning and that the provisioning of ecosystem services is essential for human well-being. The relationship between biodiversity and ecosystem services is not straightforward, whether more biodiversity would imply more ...

PREVALENCE OF UROPATHOGENIC ESCHERICHIA COLI & STAPHYLOCOCCUS AUREUS AMONG PATIENTS WITH CASES OF URINARY TRACT INFECTIONS ATTENDING BENUE STATE UNIVERSITY TEACHING HOSPITAL, MAKURDI

ABSTRACT In the present study, we investigated the prevalence of few bacterial organisms associated with urinary tracts infection and their antimicrobial susceptibility patterns. Total of Ninety (90) midstream urine samples were collected from outpatients with respect to their age group, gender and marital status attending health facility in Benue State University Teaching Hospital Makurdi. Each 0.1ml of urine sample was cultured on MacConkey agar and colonies showing lactose fermentation we...

Production of protease enzyme from fish guts using Pseudomonas fluorescens, Enterobacter cloacae and Bacillus megaterium.

Food industry wastes are an essential environmental contamination source. A large number of microorganisms including bacteria, yeast and fungi produce different types of enzymes like protease, lipase, chitinase, chitosan and ligninolytic enzyme having high biotechnological interest such as food processing, detergent, textile, pharmaceutical products, and medical therapy. Microbial synthesis of enzymes has been reported to be influenced by various factors such as carbon sources, nitrogen sourc...

Incidence of Staphylococcus aureus in Street Vended Food Sold in Calabar Municipality Nigeria

Street vended foods are the major source of food borne diseases in most parts of sub-Saharan Africa including Nigeria. In this study, six samples each of six different vended foods obtained in Calabar municipality were evaluated for bacteriological quality and presence of Staphylococcus aureususing standard microbiological techniques. Each food was examined macroscopically for evidence of spoilage, followed by culture isolation for Staphylococcus aureus. The organisms isolated were subjected ...

A COMPARATIVE STUDY OF THE ANTIBACTERIAL ACTIVITIES OF MEDICATED SOAPS ON SOME SELECTED BACTERIA FROM THE SKIN

Four medicated soaps (Ghana soap, Tetmosol, Beneks’, and Crusader) were investigated for their antibacterial activities against Staphylococcus aureus and Staphylococcus epidermis. A total number of thirty (30) students of Western Delta University, Oghara, Delta state, 15 males and 15 females were skin swabbed with sterile swab sticks. Identification of the bacterial species was by standard microbiological techniques. Crusader soap had the mean highest antibacterial activity (15.3 mm) agains...

In vitro Assessment of Selected Antibiotics, Crude Extract of Dalbergia latifolia Leaf and Their Combination on MDR Salmonella enterica Strain.

Aims: To investigate the susceptibility pattern Salmonella enterica strains to selected antibiotics and extract of Dalbergia latifolia as well as their combinatory effect on (multi-drug resistant) MDR salmonella.  Place and Duration of Study: Faculty of Pharmacy, University of Ibadan, Nigeria from October 2012-May 2013. Methodology: In this study, a total of 11 clinical isolates of Salmonella enterica strains were screened in vitro against five antibiotics (ampicillin, amoxycillin, chloramph...

Multidrug Resistant Salmonella enterica Strains in South Western Nigeria: Prevalence and Susceptibility to Ceftriaxone

Aims: To investigate the prevalence and susceptibility pattern Salmonella enterica strains to ceftriaxone in southwestern Nigeria. Place and Duration of Study: Faculty of Pharmacy, University of Ibadan, Nigeria from November 2012-May 2013. Methodology: Isolates of Salmonella enterica were characterized by established standard cultural and biochemical tests and was screened in-vitro for their sensitivity to different antibiotics (ampicillin, amoxicillin, chloramphenicol, cotrimoxazole and ceft...

CULTIVATION OF PLEUROTUS PULMONARIUS ON SAWDUST

ABSTRACT Pleurotus species are popularly known as oyster mushroom which are regarded as one of the edible mushroom of commercial important throughout the whole world. Utilization of this depends on the ability to secrete cellulolytic and hemilocellulolytic enzymes which can make use of wide spectrum of Agro industrial wastes for the growth and frutification. In this study potato dextrose agar was the only growth medium used to prepared the mycelia of P. pulmonarius. Then the culture was tran...

ISOLATION AND IDENTIFICATION OF MICROORGANISMS RESPONSIBLE FOR SPOILAGE OF DAIRY PRODUCT (MILK, YOGHURT AND LOCALY PREPARED CHEESE)

ABSTRACT Milk, Yoghurt and Cheese are highly valuable food which are readily digested and have high concentration of nutrients which have proved to be a heaven of microbes, studies were conducted on the isolation and identification of microbes [Fungi and Bacteria] on peak canned milk, yoghurt, locally prepared cheese and cheese water. It was revealed that yoghurt was devoid of microbes while milk, locally prepared cheese and cheese water harboured microbes. Locally prepared cheeses, cheese ...

Microbiology of Meat Spoilage

This paper is titled 'Microbiology of Meat Spoilage'. It clearly explains those Spoilage microorganisms associated with the Spoilage of meat and meat products and also the preservative method to be used in preserving meat. On the basis of its high moisture content and presence of adequate nutrient, meat is regarded   as   the   most   perishable   product   (Magnus,   1986)   and   thereby   highly susceptible   to   spoilage   by   microbial proliferation.  Apart...

INCIDENCE STUDY OF ROTAVIRUS ANTIGEN IN DIARRHEIC STOOL OF CHILDREN ATTENDING SABANA SPECIALIST HOSPITAL IN GOMBE, NIGERIA

ABSTRACT  Rotavirus is a common cause of diarrhea in children. This study was carried out to determine the prevalence of rotavirus antigen in children below five years presenting with diarrhea in Gombe State. Thirty (30) stool samples were analysed using ELISA KIT. Out of the total number of samples analyzed, a prevalence of 6.67% was recorded all of which are between the age group 7-12 months. A more comprehensive survey is recommended to depict true states of rotavirus as an etiology o...

ISOLATION AND MOLECULAR IDENTIFICATION OF PAENIBACILLUS SPECIES FROM PIG FARMS IN OGUN STATE, NIGERIA

ABSTRACT Paenibacillus species are opportunistic pathogens that tend to cause infections in immunocompromised individuals, it is increasingly becoming implicated in human infections but its role in animal farms is not well elucidated, this study is therefore aimed at determining the prevalence of this organism in farm animals, its molecular characterization as well as its antibiotic sensitivity profile. A total of 138 fecal samples were analyzed using standard aerobic and anaerobic culture me...

Isolation and Characterization of Aeromonas SPP. from Amarantus SPP. and Irrigation Water

ABSTRACT This study was carried out to isolate, characterize and identify Aeromonas spp. from Irrigation water and vegetables. Water and vegetable samples were collected and analyzed for physicochemical and microbiological analysis.  Physicochemical parameters such as pH, Temperature, Total Dissolved Solid (TDS), Dissolved Oxygen and Biological Oxygen Demand were determined using standard methods for water samples only. Microbiological parameters such as Total Coliform count (TC), Faecal ...


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