Microbiology Research Papers/Topics

Antimicrobial Activity of Nigella sativa Seeds Extract Against Carbapenem and Colistin Resistant Gram Negative Bacilli in Khartoum State

Abstract Introduction: One of the most serious issues in medicine is increasing resistance of Gram negative bacilli to antimicrobial agents especially broad spectrum antibiotics such as carbapenems and colistins. This fact is associated with higher mortality and morbidity rates, prolonged hospital stays and increased treatment related costs.Medicinal plants appeared to be the best alternative source for new antimicrobial drugs.. Nigella sativa is an herbaceous plant whose seeds have beenused ...

Antimicrobial and Antioxidant Activity of Hibiscus sabdariffa (Roselle) against Selected Microorganisms

Introduction: Prevalence of resistant microbial strains is increasing with time; due to the extensive and indiscriminate use of the broad spectrum antimicrobial drugs that render the current used antimicrobial agents insufficient to control some microbial infections. Objective: The present study was aimed to investigate the antimicrobial and antioxidant potential of methanolic extract of the medicinal plant Hibiscus sabdariffa L. Method: The agar well diffusion technique was used to determin...

Isolation, Identification and Antimicrobial Susceptibility Test for Various Bacteria Present in Blood

ABSTRACT  Bacteremia is one of the most significant public health concerns and is a challenge in critical care of a patient. Early diagnosis of bacteremia could be vital step in estimating appropriate treatment as well as beginning of it. The current study aims  to discover the pervasiveness of different bacterial species responsible for causing  bacteremia around the population of various gender and age group and enabling the doctors for choosing the verifiable antimicrobials through...

Isolation and Sensitivity of Bacteria Isolate from Vaginal Discharge to Antibiotics

ABSTRACT Normal vaginal flora contains a wide range of microorganisms. Bacterial vaginosis BV is the main reason for vaginal discharge. Many gram-positive and gram-negative rods i.e. E.coli, Klebsiella, Proteus, Acinetobacter and Pseudomonas spp. are major contributors to bacterial vaginosis. Aim: The present study was conducted to elucidate the frequency of various bacteria in high vaginal swabs and the sensitivity pattern of bacteria to antibiotics that are currently used Material and Metho...

Phenotypic Detection of Extended Spectrum Beta Lactamases Producing Organism Among Godfrey Okoye University Students

ABSTRACT Extended-spectrum beta-lactamases (ESBL) are enzymes that confer resistance to most beta-lactam antibiotics, including penicillin, cephalosporin, and the aztreonams. The aim of this present study is to phenotypically identify and establish the presence of ESBL-producing organism among students in the university community. Within the University community of Godfrey Okoye University, Enugu, early morning urine samples of midstream-catch were collected into sterile bottles from sixty (6...

Antimicrobial Efficacy Of Guava (Pisdium Guajava) And Orange (Citrus Sinensis) Stems Extract Against Streptococcus Mutans And Candida Albicans

ABSTRACT   This research was conducted in Microbiology Laboratory of Godfrey Okoye University to determine the antimicrobial efficacy of aqueous, methanol, ethanol of Pisdium guajava and Citrus x sinensis on the pathogenic Streptococcus mutans and Candida albicans. The Dimethyl sulfoxide was used for dissolving the plant extracts. Pisdium guajava showed antimicrobial activity against C. albicans with the exception of methanol showing no zone of inhibition to any of the isolates. The aqueous...

Extended Spectrum Beta-Lactamase in Clinical Isolates of Escherichia Coli and Klebsiella Pneumoniae from The Tamale Teaching Hospital

ABSTRACT BACKGROUND: Extended Spectrum Beta-Lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniae are pathogens of significant public health interest to which new antibiotics therapies are urgently needed. AIM: This study was designed to determine the prevalence of ESBLs in clinical isolates of E. coli and K. pneumoniae from patients attending the Tamale Teaching Hospital (TTH). METHODOLOGY: The study was conducted from April through June, 2015. A total of 140 isolates of E....

Antibody Responses to Variant Surface Antigens and Biomarkers of Endothelial Damage and Repair in The Development of Cerebral Malaria

ABSTRACT Background: The pathogenesis of cerebral malaria (CM) is still recognized as multifaceted, despite the plausible explanation provided by the high degree of sequestration of parasitized erythrocytes in major organs of the body. Postmortem studies have demonstrated the presence of hemorrhage, deposition of fibrin and clots in blood vessels of the body where sequestration has been observed. These findings support a potential association between sequestration, endothelial damage and re...

Limnological Study On Microalgal Flora Of Nakane Lake Of Dhule District Of Maharashtra, India

 Abstract:  Algae Play vital role in aquatic ecosystems by forming the energy base of the food web for all aquatic organisms. Thepresent study with relation to Potential of Cyanophyceae, Chlorophyceae, Bacillariophyceae, Euglenineae, classes of algae carried out for Nakane Lake of Dhule district. In Maharashtra very few workers have paid attention on limnological aspects of algae from lotic & lentic water habitat. These studies of Nakane Lake were carried out during December�...

Isolation and Identification of Solvent Producing Clostridia from the Soil Environment within Jos Metropolis, Nigeria.

Abstract Indigenous bacteria in the soil environment can produce a wide range of solvents and metabolites more efficiently. This study was carried out to screen Clostridium species isolated from the soil environment within Jos metropolis for solvent production. A total of 30 samples were collected from farmlands, nursery gardens, floral gardens and waste disposal sites. The pH, temperature and moisture content at various locations was determined. The mean temperature of the soil ranges from 1...

Antibiotics Susceptibility Pattern of Different Bacteria Associated With Wound Sepsis (A Case Study of University of Ilorin Teaching Hospital)

TABLE OF CONTENT Tile Page                                                                                                         i Approval Page                                                                                                ii Dedication                       �...

Influence of Different Nitrogen Sources on Growth and Phb Production of Bacterial Isolates

ABSTRACT Polyhydroxybutyrate (PHB) are biodegradable materials, which are accumulated to store carbon and energy in various microorganisms.                                    Soil samples used for the study were collected from groundnut farm garden. The isolates were screened for PHB production using sudan III stain as well as submerged fermentation. Four (4) of the best PHB producing bacteria were selected for further study. A total of twenty one (21) bacte...

Evaluation of Power Density of Ray Emissions From Gsm Masts in Selected Locations of Ilorin Metropolis

ABSTRACT The evaluation of power density of ray emissions from GSM masts in selected locations within Ilorin metropolis were carried out. The measurements taken in each spot were done using Acoustimeter. The location that shows the highest peak measurement (5.12 ± 1.07) location H, which exceeded the international approved exposure limits which is 4.1mW/m2. This research shows that there are some areas within Ilorin metropolis where these GSM antennas are installed and is emitting high radi...

Microbiology of Polyethylene-Packaged Sliced Watermelon (Citrullus lanatus) Sold by Street Vendors in Nigeria

Abstract Ten packaged, freshly sliced watermelon were collected from different street vendors to determine their microbiological quality. Eight different microbial isolates were obtained from the sliced watermelon samples, namely Escherichia coli, Klebsiella aerogenes, Proteus mirabilis, Staphylococcus aureus, Lactobacillus spp., Saccharomyces cerevisiae, Rhizopus stolonifer and Mucor spp. The effects of high density polyethylene (HDP) and low density polyethylene (LDP) packaging bags on the ...

The Assessment of Floral Abundance and Composition of Neni-Nimo Watershed in Anaocha L.G.A. of Anambra State, Nigeria

Abstract The research was carried out on the floral composition of Neni-Nimo watershed between November 2019 and July 2020. The research work aimed to find out the various plant species that inhabit the watershed, their abundance measures and importance values. In this study, the watershed was divided into three catchment areas, the upper, the middle and the lower catchment. Each of the catchment zones was divided into component parts, with an area of (50 m X 30 m) each. The experiment was la...


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