Pharmaceutical Microbiology Research Papers/Topics

Assessment of Clofazimine and TB47 Combination Activity against Mycobacterium abscessus Using a Bioluminescent Approach

Abstract Mycobacterium abscessus is intrinsically resistant to most antimicrobial agents. The emerging infections caused by M. abscessus and the lack of effective treatment call for rapid attention. Here, we intended to construct a selectable marker-free autoluminescent M. abscessus strain (designated UAlMab) as a real-time reporter strain to facilitate the discovery of effective drugs and regimens for treating M. abscessus. The UAlMab strain was constructed using the dif/Xer recombinase sys...

ANTI-BACTERIAL EFFECTS OF THE CRUDE EXTRACTS OF PHYTOLACCA DODECANDRA L. AND SCHINUS MOLLE L. LEAVES ON SELECTED HUMAN PATHOGENIC BACTERIA

Abstract: Plants possess a wide variety of secondary metabolites which have been found to exhibit antimicrobial, antioxidant and anti tumor activities. In Ethiopia, a number of plant species are traditionally used for the treatment of many diseases. Among these, Phytolacca dodecandra and Schinus molle which are abundantly available in many areas of the Country and they are widely known for their traditional medicinal values. This study was conducted with the objective of evaluating crude ext...

THNOBOTANICAL STUDY OF MEDICINAL PLANTS OF TULO WOREDA, WEST HARARGE ZONE, OROMIA REGION, ETHIOPIA

Abstract: Ethnobotanical studies are useful in documenting, analyzing and communicating knowledge and interaction between biodiversity and human society. The objective of this study was to conduct ethnobotanical study and document medicinal plants and indigenous knowledge in Tulo district. Ethnobotanical data was collected from December 2019 to January 2020 using semi-structured interview, focus group discussion and direct field observation with 80 randomly selected non traditional healers a...

Investigating the anti-filarial efficacy and molecular interactions of thiadiazol derivative: Insight from quantum chemical calculations, pharmacokinetics, and molecular docking studies

Abstract Parasitic infections such as lymphatic filariasis (elephantiasis) solely result from the infestation of a slender and thread-like worm. This condition precipitates swelling, often accompanied by skin alterations, potentially leading to substantial disability and disfigurement. Recent advancements in preventive measures for lymphatic filariasis encompass mass drug administration (MDA) involving antifilarial drugs. To that effect, this investigation employs an experimental and theoreti...

Investigating the anti-filarial efficacy and molecular interactions of thiadiazol derivative: Insight from quantum chemical calculations, pharmacokinetics, and molecular docking studies.

Abstract Parasitic infections such as lymphatic filariasis (elephantiasis) solely result from the infestation of a slender and thread-like worm. This condition precipitates swelling, often accompanied by skin alterations, potentially leading to substantial disability and disfigurement. Recent advancements in preventive measures for lymphatic filariasis encompass mass drug administration (MDA) involving antifilarial drugs. To that effect, this investigation employs an experimental and theoret...

Formulation and Evaluation of Some in Vitro and in Vivo Properties of Artemether-Loaded Self Emulsifying Drug Delivery System

ABSTRACT The aims of this research work were to formulate artemether-loaded self emulsifying drug delivery system (SEDDS), evaluate some of its in vitro and in vivo properties, and prevent drug precipitation through supersaturation approach. The solubility of artemether (ARM) in various oils, surfactants and cosurfactants was determined using the equilibrium solubility method. Vehicles such as Triacetin®, Labrasol® and Transcutol P® were selected as components of the formulation due to the...

Evaluation of the Antinociceptive and Anxiolytic-Like Activities of Leaf Extract and Fraction of Phyllanthus Muellerianus Kuntze

ABSTRACT The anxiolytic and antinociceptive properties of the leaf extract and fraction of Phyllanthus muellerianus Kuntze (Phyllanthaceae) were investigated.The extract (MME; 500.18 g; 10%w/w), obtained by cold maceration in 1:1 mixture of methylene chloride: methanol for 48 h, was fractionationed in a silica gel column which yielded the ethyl acetate fraction (EF; 199.2 g; 39.83% w/w). The extract and fraction were subjected to phytochemical analysis. Acute toxicity of MME was determined in...

Physico-Technical and Tableting Properties of Grades of Microcrystalline Cellulose Derived from Sorghum and Andropogon Plants

CONTENTS Page Acknowledgement ........................................................................................................... iv Abstract ................................................................................................................................. vi List of Tables .................................................................................................................... xiv List of Figures .................................................................

Phytochemical and Bioactivity-Guided Evaluation of the Antibacterial Constituents of Psidium Guajava (Linn.) and Loranthus Micranthus (Linn.) Leaves

ABSTRACT Antibiotics have remained the mainstay of drug therapy of infectious diseases worldwide. Their use is, however, limited by their numerous adverse effects and rapid development of microbial resistance. Identification of natural products from plants that may serve as valuable sources of antimicrobial agents for medicinal or agricultural uses seems to be a viable alternative to the conventional antibiotics. To achieve this goal, biological assays should be carried out in order to identi...

Comparative study of the anti-eczema and antifungal activities of Mitracarpus scaber with Sulphur ointments, Hydraulic fluid and Skineal on eczematous skin

Abstract Eczema, an atopic dermatitis is one of the most economically important skin disorder, therefore remedial attention needs to be given to it. Unlike the orthodox belief, eczema is a genetic disorder of the epidermis (filaggrin, CARD 11 or KIF3A gene mutation) not an infection, therefore, treatment must be targeted towards correcting this rather than targeting Candida albicans, the yeast that infects the eczematous skin as a result of environmental exposure. Currently, all types of chem...

Evaluation Of The Immunomodulatory Activity Of Hoslundia Opposita Vahl (Lamiaceae) Leaf Extract.

ABSTRACT The immunomodulatory properties of methanol extract and fractions of Hoslundia opposita leaf on Delayed-Type Hypersensitivity (DTH), primary and secondary humoral response and in vivo leucocyte mobilization were evaluated. Acute toxicity test of crude extract and phytochemistry study was carried out. The methanol extract (ME) at 200, 400 and 800 mg/kg body weight produced significant (P

Effects Of Heavy Metals On The Air Pollution Tolerance Indices ( Apti ) Of Five Medicinal Plants Growing Within Quarry Site In Ishi-Agu, Ebonyi State, Nigeria.

ABSTRACT Effects of heavy metals on the Air Pollution Tolerance Indices (APTI) of five medicinal plants growing within quarry site in Ishi-Agu, Ebonyi State, Nigeria were studied. Following laboratory analysis of the leaves of the plants for metal load and four biochemical parameters of APTI computation. The metal load was determined by the Atomic Absorption spectrophotometric method, while the ascorbic acid was determined by the titremetric method, leaf extract pH by the electro-chemical te...

Molecular Detection Of Extended- Spectrum Beta-Lactamases And Mcr-1 Genes In Gram Negative Isolated From Patient Of Urinary Tract Infections In Khartoum State

Abstract This study aimed to detect extended specterum beta lactamase genes in Gram negative bacteria associated with Urinary Tract Infections in Khartoum State. Ninty one urine specimens were collected in this study. Samples were cultured on CLED agar and bacteria were identified by the conventional biochemical methods. Modified Kirby-Bauer method was usedfor sensitivity testing by using the following antibiotics: Gentamicin, Ciprofloxacin, Co-trimoxazole, Imipenem and Ceftazidime. A total ...

Antifungal Capacity of Lactic Acid Bacteria Isolated From Salad Vegetables

ABSTRACT This study explores the use of lactic acid bacteria from fresh salad vegetables to inhibit fungal growth. The antifungal assay was done using the agar well diffusion method as reported by Schillinger and Lucke (1989). The largest zone of inhibition (25mm) was recorded by the antagonistic activity of the isolate identified to Lactobacillus plantarum against Candida albicans ATCC 90029. No activity was recorded against Candida parapsilosis ATCC 22019, C. valida UCH 1508, C. pseudotrop...


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