PHYTOCHEMICAL ANALYSIS AND ISOLATION OF COMPONENTS OF STEM-BARK OF CHRYSOPHYLLUM ALBIDUM (G.Don)

Chrysophyllum albidum (Sapotaceae) is known to possess bioactive compounds belonging to important phytochemical groups. This work aims at exploring the bioactive constituents of the stem-bark of Chrysophyllum albidum, and further investigating their phytochemical compositions. Stem bark of Chrysophyllum albidum was collected, dried, powdered and extracted with hexane for 13 hours using soxhlet extraction. This was concentrated using a rotary evaporator. Phytochemical screenings of the extract were performed using standard tests. The crude extract from the stem bark was subjected to column chromatography. Fractions (250 ml each) were collected and concentrated under pressure using rotary evaporator. Pooled fractions were further purified using micro-column. The isolated constituents were subjected to ultraviolet and infrared spectroscopic analysis. From the column chromatography of the crude extract, two constituents were isolated. Phytochemical analysis of the extract revealed the presence of alkaloid, steroid, saponin, tannin, reducing sugar and flavonoid. The presence of these secondary metabolites in the stem-bark might be the reasons for its use in the treatment of yellow fever and malaria.