Biotechnology Research Papers/Topics

HYDROPONIC GROWING OF LETTUCE (Lactuca sativa L.) AND PRODUCTION OF BIOORGANIC LIQUID FERTILIZER FROM CHICKEN MANURE AND ONION PEELS.

Abstract: Lettuce (Lactuca sativa L.) is one of the most popular leafy salad vegetables for fresh consumption and containhealth-promoting compounds.Hydroponic method is a techniques of cultivation plants using bioorganic liquid fertilizer without soil and is more important than soil because of efficient resourcesmanagement and quality food production. This study,wasaimed to produce bioorganic liquid fertilizer from fermented of fresh chicken manure and onion peels that can be used for hydrop...

Biogas Production from Wheat Straw Co-Digested With Poultry and Goat Manure

Abstract: Biogas production using anaerobic digestion of organic waste materials provides an alternative environmentally friendly renewable energy. In this study, the production of biogas from co-digestion of wheat straw with poultry and goat manure was evaluated in seven treatment under mesophilic condition 380C for30 days of retention time, using a batch digester in Botanical and Central Laboratories of Haramaya University. Total solids, volatile solids, percent moisture content, pH, organ...

IN VITRO PROPAGATION OF SELECTED SUGARCANE (Saccharum officinarum L.) VARIETIES (C 86-12 and C 86-165) THROUGH APICAL MERISTEM

Abstract: Sugarcane (Saccharum officinarum L.) is monocotyledonous crop plant that belongs to the Poaceae family that mostly propagates through conventional methods. However, conventional propagation lacks rapid multiplication procedures to commercialize newly released varieties within a short period of time. Hence, the objective of this work was to optimize in vitro micro propagation protocol for two sugarcane varieties (C 86-165 and C 86-12) using shoot apical meristem explants. The two va...

PRODUCTION OF BIOGAS FROM AN INVASIVE WEED BIOMASS (Argemone ochroleuca L.) CO-DIGESTED WITH COW MANURE UNDER ANAEROBIC CONDITON

Abstract: Biogas is an alternative and renewable energy source produced through anaerobic digestion of organic matter whereby the organic matter is converted into a combustible gas rich in methane (CH4),solid residue(sludge) and a liquid effluent. The aim of this study was production of biogas from Argemone ochroleuca co-digested with cow manure under mesophilic conditions (38ºC) using batch digester for 30 days of fermentation time. The experimental design was completely random design with...

RODUCTION AND PARTIAL CHARACTERIZATION OF LACCASE FROM OYSTER MUSHROOM (Pleurotus ostreatus) COLLECTED FROM THE BARK OF EUCALYPTUS TREE

Abstract: The aim of the current study was to evaluate the effect of different growth substrates on laccase production and to partially characterize it in terms of the effect that pH, temperature and substrate concentration had on its activity. The study was conducted at the National Agricultural Biotechnology Research Center, Holetta. The fruiting bodies of Pleurotus ostreatus were collected from live old Eucalyptus tree barks. The potential of Pleurotus ostreatus in laccase production was ...

Biogas Production from Co-Digestion of Goat Manure and Watermelon (Citrullus Lanatus) Fruit Peels Under Anaerobic Condition

Abstract: Biogas is an alternative source of energy that is produced by methanogenic bacteria through the bio-degradation of organic material under anaerobic conditions. In this study, biogas production from co-digestion of the goat manure (GM) and watermelon peels(WMP) in five mix ratios were evaluated under 38ºC using batch fermentation. In all treatments, physico-chemical parameters such as total solid (TS) and volatile solid (VS), organic carbon, and pH were measure before and after dig...

Isolation and Characterization of Rhizobia from Leguminous Plants and Assessing their Plant Growth Promoting Properties

Abstract: The increasing world population led to mass application of chemicals in agriculture to increase food production and meet the demand. Today, attentions are diverted into biofertilizers than the chemical fertilizers. This study was initiated to isolate and characterize rhizobia from Faba bean (Vicia faba L.) and assessment for plant growth prompting substance. Faba bean samples were collected by systematic random sampling from farmers’ fields of three geographically varied location...

Invitro Regeneration of Echinops Kebericho from Shoot Explants

Abstract: Echinops kebericho is an erect massive rootstock bearing perennial herbs or shrub belonging to the family Asteraceae. Echinops kebericho, endemic to Ethiopia is a critically endangered medicinal plant. It is among few important medicinal plants of the country valued primarily for root parts. The seeds of Echinops kebericho characterized by short viability period and lose their capacity to germinate very quickly. This study aimed to develop in vitro regeneration of Echinops keberich...

ISOLATION AND SCREENING OF SELECTED HEAVY METAL RESISTANT BACTERIA FROM KOMBOLCHA TANNERY INDUSTRY EFFLUENT

Abstract: This study was aimed to replace the chemical and physical treatment with biological treatment to save the environment. It was concerned with the isolation, screening, and charact erization of heavy metal-resistant bacteria isolated from Kombolcha tannery industry effluent. Different ways were used to select the maximum of isolates resistant to heavy metals. Based on, morphological, gram stain and biochemical tests six resistance bacteria were isolated by using free nutrients agar m...

THE IMPACT OF TEMPERATURE PRETREATMENT ON BIOGAS YIELD FROM SAWDUST, CO-DIGESTED WITH COW DUNG

Abstract: Renewable resources play a significant role in reducing carbon dioxide emissions around the world, and with this in mind, biomass and waste is a candidate to be one of the most widely utilized renewable energy resources. Therefore, this research was conducted to investigate the production of biogas from saw dust, cow dung in solo or mix through anaerobic digestion with five treatment ratios, under mesophilic condition (38ºC) using batch digesters at Haramaya University for 30 days...

BIOETHANOL PRODUCTION VIA FERMENTATION OF BANANA PEEL USING Saccharomyces cerevisiae

Abstract: Bioethanol derived from biomass contributed 10 to 14% of the total world energy supply and solved the world crisis such as global warming and depletion of fossil fuels. Therefore, this research is initiated to produce ethanol from biomass such as banana peel which was fermented bcy different concentration of substrates with 0.5% and 1% yeast in different fermentation period’s day, with the aim of optimizing the substrate concentration for ethanol production. The sample (Banana pe...

PRODUCTION OF BIOORGANIC LIQUID FERTILIZER FROM COW MANURE AND GROUNDNUT HUSK

Abstract: Organic Liquid Fertilizer made from the decomposition of organic material such as plant residue and animal waste through anaerobic fermentation comprises number of growth substances, vitamins, antibiotics, amino acids and useful micro-organisms. The advantage of these fertilizers resolve a deficient of nutrient and provide the additionally faster nutrient supply. Thus, the present study was aimed to produce organic liquid fertilizer from cow manure and groundnut husks through aerob...

PREPARATION OF BIOORGANIC LIQUID FERTILIZER FROM CHICKEN MANURE AND GROUNDNUT HUSK

Abstract: Natural organic fertilizers used in plant cultivation are characterized by low efficiency and soil-applied in large quantities to obtain high yield. The bioorganic fertilizer obtained through anaerobic fermentation comprises number of growth substances, vitamins, antibiotics, amino acids and useful micro-organisms. Bioorganic liquid fertilizer not only increases bioorganic fertility of crops (in comparison to the control and prototype fertilizer), but also accelerates their maturat...

THE EFFECT OF CO-DIGESTION ON BIOGAS PRODUCTION WITH Bidens pilosa L. AND POULTRY MANURE THROUGH ANAEROBIC DIGESTION

Abstract: Biogas is an eco-friendly renewable energy source through the anaerobic digestion of organic waste materials. In this study, biogas production from co-digestion of Bidens pilosa(BP) and poultry Manure (PM) in five mix ratios (100%BP, 75%BP:25%PM, 50%BP:50%PM, 25%BP:75%PM and 100%PM) were evaluated under at 38ºC using a batch mode digester over 21 days of fermentation. In all treatments, physico-chemical parameters such as pH, total solid, volatile solid, moisture content, organic ...

PRODUCTION OF ALKALINE PROTEASE FROM AVOCADO (Persea americana Mill.) SEED AND PEEL WASTES USING FUNGI

Abstract: Microbial proteases are hydrolytic enzymes that are widely used in many industrial processes. This study aimed to evaluate the production of alkaline protease (AP) from fungal isolates sourced from avocado (Persea americana Mill.) peel (AVP) and avocado seed (AS) under solid state fermentation (SSF). AVP and AS was collocated form Harar juice house into plastic bag and serial diltuion method was used for fungal isolatin. The isolates were subutured on potato dextrose agar (PDA) and...


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