Abstract Trebouxiophytes of the Dictyosphaerium–morphotype from inland waters of Africa were studied using a polyphasic approach of SSU and ITS rDNA phylogeny, secondary structure of the ITS and observations made with a light microscope. Although the morphological criteria for differentiating species and genera are scarce, the genetic diversity of these algae was very high. Based on our genetic analysis findings, we described four new genera containing five new species: Compactochlorella d...
Abstract The last two decades have witnessed increasing episodes of lesser flamingo die-offs in East Africa. Based on data on phytoplankton composition, biomass, and flamingo population density in three alkaline-saline lakes of Kenya (Bogoria, Nakuru, and Oloidien) in 2001–2010, this study explored the link between sudden flamingo deaths and fluctuations in algal food quantity and quality. The phytoplankton biomass ranged from 13 to 768 mg Æ L)1. Similarly, flamingo numbers varied widely ...
Abstract PCR-based denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene fragments was used to identify the cyanobacterial phylotypes in sediments and plankton of saline–alkaline and freshwater lakes of Kenya. The detection of the aminotransferase domain located on modules mcyE and ndaF using specific molecular markers confirmed the presence of potential toxin-producing cyanobacteria. The eight nucleotide sequences obtained from DGGE bands were placed in three divergent ...
Abstract Cyanobacterial mats at hot springs on the shore of the alkaline Lake Bogoria, Kenya, were investigated regarding species community and cyanobacterial toxin content. The hepatotoxins microcystin-LR, -RR, -LF and -YR, and the neurotoxin anatoxin-a were present. The mats were dominated by Phormidium terebriformis, Oscillatoria willei, Spirulina subsalsa and Synechococcus bigranulatus. The concentration of microcystins in mat samples, ranged from 221 to 845 microg microcystin-LR equival...
Abstract The food web of the saline–alkaline lakes of East Africa is characterised by a unique interaction between the Lesser Flamingos as consumer birds and the cyanobacterium Arthrospira fusiformis as the primary producer. However, this interaction is disturbed frequently by alterations of the phytoplankton community. During the period 2001–2012, species of the cyanobacteria Anabaenopsis and Cyanospira were observed in four soda lakes of the African Rift Valley and compared to the enti...
Abstract Temporal changes in phytoplankton chlorophyll a, composition, diversity, biomass (density and fresh weight) and primary production were investigated at the Turkwel Gorge Reservoir (Kenya) over a two year period (1994 and 1995). The phytoplankton properties investigated revealed a seasonal pattern that was very distinct in 1994 and muted in 1995. The wet season was characterized by higher levels of chlorophyll a, biomass and primary production and a lower diversity. A prominent seaso...
Abstract In the present study, significative HAB’S bloom was confirmed in Matanchén Bay during Febrary 2003. The Raphidophice Fibrocapsa cf. japonica, Toriumi et Takano 1973 (=Chatonella japonica) has dominated (97%) red tide event occurring lately in coastal waters of Nayarit state, with high cell concentration of 4-6 millions of cells per liter of water. The duration of this bloom could not be determined exactly due to limiting sampling. There have been no reports of fish kills or human...
Abstract Kenya is one of the high endemic zones for hepatitis B virus (HBV) infection. The consensuses on prevalence of the HBV genotypes and the existence of their variants have not been fully established in Kenya. Hence, there is a need to further monitor the diversity of HBV. This study aimed to extend the current molecular and epidemiological information about the geographical distribution of HBV genotypes and subgenotypes, as well as to describe the hepatitis B surface antigen (HBsAg) v...
Abstract BACKGROUND: Development of “combination” assays detecting in parallel, within a single test, Hepatitis C Virus (HCV) antigens and antibodies, not only reduces the window period in HCV-infection but also costs. Reduction of costs is important for developing countries where money and personal resources are limited. METHODS: We compared the Monolisa® HCV Antigen-Antibody Ultra (Bio-Rad Laboratories Limited, Marnes La Coquette, France) with the AXSYM HCV version 3.0 (Abbot Diagnost...
Abstract High yield is a major goal for watermelon (Citrullus lanatus) breeders. The objective of this study was to compare the yield components of some watermelon accessions available in Kenya and to identify high yielding accession(s) for recommendation to Kenyan growers and for use in breeding programs. A field trial involving five cultivated watermelon accessions in Kenya namely ‘Sugarbaby’, ‘Crimson Sweet’, ‘Charleston Gray’, ‘Yellow Crimson’; and one local landrace (GBK...
Abstract Riverine ecosystems in the Lake Victoria Basin (LVB) significantly contribute to the lake’s water budget and aquatic biodiversity. Despite their significance to the lakes fisheries, only limited biodiversity studies have been carried out, particularly in the smaller riverine ecosystems. This study investigated the fish species composition, diversity and distribution in two small riverine ecosystems (Rivers Awach Seme and Kisian) draining the Kenyan Lake Victoria Basin. Sampling wa...
Abstract Woody debris accumulations are important ecological components in low order forested streams. They provide forage and refuge for a macroinvertebrates, which are key trophic components for riverine fish. The occurrence of woody debris in tropical streams is however threatened by large scale destruction of catchment forests thereby threatening the riverine biodiversity. Despite their importance, the current status of woody debris accumulations in tropical forested streams is unknown. ...
Abstract Utilization of mushrooms collected from the wild requires adequate description of useful phenetic features and domestication protocols. This study investigated morphological characters and spawn production procedures of three Kenyan native strains of wood ear mushroom [Auricularia auricula (L. ex Hook.) Underw.]. Nine basidiocarps were selected from collections made in three forest reserves within Kakamega Forest in Western Kenya and morphologically characterized. Mycelia were raise...
Abstract Genetic diversity and relatedness were assessed among three most common commercial watermelon cultivars in Kenya; one newly introduced commercial cultivar from the U.S., one Kenyan landrace and one wild (Citrullus colocynthis) accession. The six accessions were grown in the field for two seasons under sub humid tropical conditions. Randomized Complete Block Design (RCBD) with three replications was used. Data was collected on morphological features of watermelon which include vine, ...
Abstract Actinobacteria are ubiquitous and have been detected in terrestrial and aquatic ecosystems. Although, various studies have focused on the ecology of this phylum, data on the ecology of actinobacteria endemic to soda lakes are scanty. This study aimed to determine actinobacterial diversity in Lake Magadi. Four different sampling points were selected randomly within the lake to cover a broad range of sample diversity. Wet sediments and water samples were collected from each sampling p...