Natural & Applied Sciences

Natural & Applied Sciences Research Papers/Topics

The relative contributions of host density and genetic diversity on prevalence of a multi-host parasite in bumblebees

Abstract: The role of population and demographic factors in influencing the transmission and establishment parasites infect-ing multiple hosts is poorly understood. We assessed the effects of these factors on parasite prevalence in a model system – the intestinal protozoan Crithidia bombi (Trypanosomatidae) infecting a range of bumblebee species (Bombus spp.). We used microsatellite markers and sibship reconstruction to infer genetic diversity and the density of host populations to infer t...

Effect of alternative rearing substrates and temperature on growth and development of the cricket Modicogryllus conspersus (Schaum)

Abstract: The increasing demand for animal protein in sub-Saharan Africa has led to insects being considered an alternative protein source for food and feed. In Uganda, chicken mash, containing fish meal as the protein source is commonly used to rear crickets. However, fishmeal is an expensive protein source that is also human food. This study therefore aimed at establishing an alternative preferred (consumed in the highest quantity) plant leaf diet for raising the Modicogryllus conspersus c...

The period of insect research in the tropics: a bibliometric analysis

Abstract: The International Journal of Tropical Insect Science (Int J Trop Insect Sci) is a peer-reviewed journal established in 1980 to promote insect science mainly in the tropics. This study aimed to provide a Bibliometric overview of Int J Trop Insect Sci publications and citations between 2012 and 2020, ending September 2020. A sample of 488 documents extracted from the Web of Science (WoS) was analysed using widely used bioclimatic indicators. The articles were written by 1726 authors....

Characterization of honeys produced by sympatric species of Afrotropical stingless bees (Hymenoptera, Meliponini)

Abstract: We investigated the effect of bee species identity and harvesting methods on the chemical composition and antiradical activity of 53 honey samples, produced by six stingless bee species in western Kenya (Kakamega forest). Our results illustrate that none of the assayed parameters significantly varied between the honey samples harvested by “punching holes” (n = 25) and “squeezing” (n = 28) methods. By contrast, species identity drove significant differences in the assayed pa...

Diversity and phylogenetic analysis of endosymbionts fromTrioza erytreae(Del Guercio) and its parasitoids in Kenya

Abstract: The African citrus triozid (ACT), Trioza erytreae (Del Guercio), is the primary vector of Candidatus Liberibacter africanus (CLaf), the causative agent of Africa citrus greening disease (ACGD). This study evaluates the diversity of ACT parasitoids and further characterizes endosymbionts associated with both T. er y treae and its parasitoids that could be used as biological control agents of T. er y treae and management of ACGD. Mitochondrial cytochrome oxidase I gene was used to re...

Genetic Diversity, Distribution, and Genomic Characterization of Antibiotic Resistance and Virulence of Clinical Pseudomonas aeruginosa Strains in Kenya

Abstract: Pseudomonas aeruginosa is a leading cause of nosocomial infections worldwide. It can produce a range of debilitating infections, have a propensity for developing antimicrobial resistance, and present with a variety of potent virulence factors. This study investigated the sequence types (ST), phenotypic antimicrobial susceptibility profiles, and resistance and virulence genes among clinical isolates from urinary tract and skin and soft tissue infections. Fifty-six P. aeruginosa clin...

Prediction of breeding regions for the desert locust Schistocerca gregaria in East Africa.

Abstract: Desert locust outbreak in East Africa is threatening livelihoods, food security, environment, and economic development in the region. The current magnitude of the desert locust invasion in East Africa is unprecedented and has not been witnessed for more than 70 years. Identifying the potential breeding sites of the pest is essential to carry out cost-effective and timely preventive measures before it inflicts significant damage. We accessed 9,134 desert locust occurrence records an...

Protein Content and Amino Acid Profiles of Selected Edible Insect Species from the Democratic Republic of Congo Relevant for Transboundary Trade across Africa

Abstract: This study analyzed the protein content of ten edible insect species (using the Dumas method), then focused on the amino acid (AA) profiles of the six major commercially relevant species using HPLC (high-pressure (or performance) liquid chromatography). The protein contents varied significantly from 46.1% to 52.9% (dry matter); the Orthoptera representative yielding both the highest protein content and the highest values in three essential amino acids (EAAs). Regarding Lepidoptera ...

Traditional ecological knowledge and non-food uses of stingless bee honey in Kenya’s last pocket of tropical rainforest

Abstract: Background Stingless bee honey (SBH) is a natural remedy and therapeutic agent traditionally used by local communities across the (sub-)tropics. Forest SBH represents a prime non-timber forest product (NTFP) with a potential to revitalize indigenous foodways and to generate income in rural areas, yet it is also used in a variety of non-food contexts that are poorly documented in sub-Saharan Africa and that collectively represent a significant part of the local traditional ecologica...

Unravelling the nutritional and health benefits of wheat bread enriched with meat powder from laying hen fed diet with insect (Hermetia illucens) meal

Abstract: Wheat bread is among stable foods that are nutritionally imbalanced, thus enrichment is crucial. We evaluated the nutritional impact of high-valued wheat bread enriched with varying levels of meat powder from hen fed diet with insect (Hermetia illucens)-based meal. Crude protein and ash in bread increased with increasing inclusion of meat powder. Limiting amino acids like lysine and threonine in enriched bread products increased by 3.0–4.5 and 1.8–3.1-folds, respectively. Omega...

Neurocognitive and mental health outcomes in children with tungiasis: a cross-sectional study in rural Kenya and Uganda

Abstract: Tungiasis, a neglected tropical parasitosis, disproportionately afects children. Few empirical studies have reported neurocognitive and mental health outcomes of children with ectoparasitic skin diseases like tungiasis. Pathophysiology of tungiasis suggests it could detrimentally afect cognition and behaviour. This study pioneered the investigation of neurocognitive and mental health outcomes in children with tungiasis

Management of Tuta absoluta with introduced and native biocontrol agents

Abstract: Tomato, Solanum lycopersicum L. (Solanaceae) is severely damaged by the South American tomato moth, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). Surveys among producers confirmed that the use of synthetic insecticides increased since the invasion of T. absoluta. The risks associated with the residual effect of insecticides in edible food and the negative effects it may have on ecosystem services,also increased. The aim of this project was to investigate the management of T. ...

Role of Grasses in Oviposition site selection by Malaria Vectors in Western Kenya

Abstract: Malaria continues to be among the top leading causes of illness and deaths in Africa. Its elimination is challenging in high transmission areas using insecticide-based intervention tools alone. Understanding the ecology and behaviour of disease vectors, including the olfactory cues, are essential for the development of novel, insecticide-free control tools. Recent work has highlighted that selected graminoid plants release volatile chemicals that attract malaria vectors. However, t...

The Population Dynamics of the Cassava Green Spider Mite, Mononychellus Tabajoa 9Bondar) (Acarina : Tetranychidae) in Relation to its Predators and Environmental Factor in Western Kenya

Description: A thesis submitted to the Faculty of Science in fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Zoology, Kenyatta University.

Occurrence and Interactions of Mycobiomes with the Anopheles gambiae Host

Abstract: Anopheles mosquitoes are colonized by diverse microorganisms that may influence hosts‟ biology and vectorial capacity. Resident Anopheles bacterial symbionts isolated from these hosts are known to influence traits such as generational fitness, survival, host seeking,and infectivity of pathogens such asPlasmodium falciparum.Eukaryotic symbionts such as fungi residing in the gut of Anopheles gambiaecould confer similar phenotypes, but are undexplored. It was hypothesized that fungi...


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