Natural & Applied Sciences

Natural & Applied Sciences Research Papers/Topics

Isolation and Identification of Some Microbial Pathogens Associated with the External Body Surface of Periplaneta americana in Umuahia, Abia State

Aim:  This  study  was  carried  out  to  isolate  and  identify  the  microbial  pathogens  of  medical importance  on  the  external  body  surfaces  of  Periplaneta  americana  collected  from  toilet  areas, residential/hostel  areas  and  health  facilities  in  Umuahia  metropolis,  Abia  State,  south-eastern Nigeria.   Study Design: Cross sectional study.  Place  and  Duration  of  Study:  The  study  was  carried  out  in  Umuahia ...

MODELING OF HEALTH RELATED QUALITY OF LIFE AMONG DIABETES PATIENTS USING INTEGRATED FUZZY INFERENCE SYSTEM AND LINEAR REGRESSIONS (1)

Health related quality of life is one of the growing subjects used for evaluating health conditions among patients who suffer from specific ailment.It has been assumed that identification of the variables is able to mirror the one's general health conditions.However,formulating the extent of contribution is multiple variable towards overall health conditions is not straight forward as the arbitrary nature of HRQL variables.This paper aims to model the relationship between HRQL variables using...

Secondary Metabolites from Mimosa Pudica: Isolation, purification and NMR Characterization

Abstract: Aim: isolation, purification and NMR characterization of secondary metabolites in Mimosa pudica. Study design: extraction, isolation and identification of the secondary metabolties from the petroleum ether and  metabolites extracts from Mimosa pudica. Methodology: In the course of our study on Mimosa pudica, a compound was isolated and purified using  different chromatographic techniques and the structure of the compound determined on the basis of their  spectroscopic data (IR, H...

Antifungal Activity of Mimosa Pudica,Isolation and NMR Characterization of Bioactive Components.

ABSTRACT  In this study, the whole plant of Mimosa pudica was extracted using absolute ethanol. The crude ethanolic extract and its isolated triterpenoid glycoside were tested for antifungal activity towards Aspergillus flavus and Trichophyton rubrum using Well Diffusion Method. At concentrations of 25 –100 mg/mL, the extract possesses antifungal activity (from being partially active to very active) against Aspergillus flavus and Trichophyton rubrum. The TLC profile of the crude extract...

Quantitative Determination of Secondary Metabolites and Antimicrobial Activity of Mimosa Pudica

Abstract: The side effects and the increasing microbial resistance to synthetic drugs in the management and  treatment of infections remain an issue in modern medicine, hence the increasing research in phytomedicine.  Therefore in the present study, the active phytocomponents of Mimosa pudica’s wholesome parts were  revealed using quantitative phytochemical analysis. The antibacterial activity of the Ethanolic extract was  studied using Well diffusion method. The activity was tested aga...

Decolourization of Textile Dye Effluent by Resident Bacterial Species

Textile dye effluents are released into the environment without any pretreatment and pollute water, to prevent contamination of our valuable resources; removal of these pollutants is of great vitality. For this purpose, waste water samples were collected from dye contaminated sites of Kofar Mata dye pits based in Kano State. Certain parameters which include temperature and pH were determined in the Laboratory. The residual bacterial load was found to be in the range of 1.4 x106 - 4.0x106 cfu....

Geoelectrical Survey of Ground Water in Some Parts of Kebbi State Nigeria, a Case Study of Federal Polytechnic Bye-Pass Birnin Kebbi and Magoro Primary Health Center Fakai Local Government

Abstract Schlumberger array is the most commonly used among other arrays for Vertical Electrical Sounding (VES) and  it requires large spacing at both ends for deeper subsurface information. The problem of limited space for spreading in built up areas could lead to incomplete information from deeper depth. The Geolectrical survey involving electrical resistivity  methods has been carried out at Birnin Kebbi and Fakai Local Government Area in Northern Nigeria with the view to delineate ...

QUALITY ASSURANCE AND QUALITY CONTROL FOR WATER AND FOOD ANALYSIS

Great attention has been paid to food and water quality for centuries. The requirements have been changed according to the development of human knowledge in the field of nutrition, chemistry, microbiology, and analysis. It is necessary first to point out that the term food and water quality is a complex one that includes many indicators. For food and water to be classified as good, it has to fulfill the requirements, especially from the nutritional, sensory, hygienic-toxicological, and techn...

Biofilm Formation and Antibiotic Resistance of Uropathogens Isolated from Patients attending a Teaching Hospital in Anambra State Nigeria

The main aim of this research was to characterize the enterobacterial uropathogens with respect to drug resistance in Awka, south-eastern Nigeria. The following enterobacterial uropathogens isolated from patients attending Chukwuemeka Odumegwu Ojukwu University Teaching Hospital, Awka were studied: E.coli (58), Salmonella spp. (15), K. pneumoniae (14), Citrobacter freundii (10) and Enterobacter aerogenes(3). Their antibiotic susceptibility patterns against major classes of antibiotics were e...

Reverse Osmosis Technology, its Applications and Nano-Enabled Membrane

Reverse Osmosis (RO) is the finest of all membrane filtration system, it is a membrane based technology to purify water by separating the dissolved solids from feed stream resulting in permeate and reject stream for a wide range of applications in domestic as well as industrial applications. The objective of this paper is to review the concept of reverse osmosis and the current developments in the RO membrane materials which are the key determinants in the process. This review paper also ...

Phytochemical Screening and Antibacterial Activity of Spondias mombin Leaves

Phytochemical investigation was carried out on the crude ethanol extracts of the leaves of Spondias  mombin. The antibacterial activity of the ethanolic and the Chloroform leaf extract was tested  against four bacteria; Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Salmonella typhi using disc diffusion method. The results indicated that both extracts showed inhibitory activity against clinical isolates of the gram negative bacteria Staphylococcus aureus, P. aerugi...

The Physicochemical Parameters and Levels of Selected Heavy Metals (Cd, Cr, Mn, Zn and Pb) in Soil around Dana Steel Rolling Limited Katsina

The  physicochemical  parameters  and  levels  of  selected  heavy  metals  (Cd,  Cr,  Mn,  Zn  and  Pb)  in soil  around  Dana  Steel  Rolling  Limted  Katsina  were  investigated  in  this  research.  The  sample  area was  divided  into  four  units;  East,  West,  North  and  South  and  label  as  A,  B,  C  and  D  respectively using  stratified  random  sampling  method.    The  soil  was  digested  using  H2SO4,  HClO4 and  H...

ANTIBIOTIC SENSITIVITY TESTING ON EGGS FROM LAYERS TREATED WITH ANTIBIOTIC

ANTIBIOTIC SENSITIVITY TESTING ON EGGS FROM LAYERS TREATED WITH ANTIBIOTIC   A RESEARCH PROJECT   ABSTRACT Antibiotic sensitivity testing is the susceptibility of bacteria to antibiotics, it is carried out to determine which antibiotic will be most successful in treating a bacterial infections. The total mean colony count of the bacterial isolates in the two set of egg sample  carried out showed the mean total bacterial count as 0.33x102 – 3.6 x 102 cfu/ml. While the antibiot...

ANTIBIOGRAM OF BACTERIAL ISOLATED FROM SEALED AND UNSEALED DRUGS

ANTIBIOGRAM OF BACTERIAL ISOLATED FROM SEALED AND UNSEALED DRUGS   A RESEARCH PROJECT ABSTRACT The study on the isolation and antibiogram of bacterial isolated form sealed and unsealed drugs revealed total Bacteria count ranging form 1.2 X 107 to 11.5 X108cfu/g for unsealed and 1.5 X107 to 9.2 x 107cfu/g  for sealed. The following bacterial isolates were identified these include Bacillus sp, klebsiellssp, staphylococcus sp Escherichia sp, Lactrbacillussp, proteussp and Citrobacter...

ANALYSIS OF TRACE METALS IN MUSCLE AND LIVER OF COW MEAT PURCHASE FROM URUA IKOT OSURUA

ANALYSIS OF TRACEMETALS IN MUSCLE AND LIVER OF COWMEAT PURCHASE FROM URUA IKOT OSURUA. A RESEARCH PROJECT ABSTRACT The concentration of trace metals (cadmium, Lead, Iron, Copper and Zinc) in two organ (muscle and liver) from cow meat obtained from urua Ikot Osurua was determined using Atomic absorption spectrophotometer. Their result was as follows muscle (Cadmium: 0.78±0.002, Lead: 0.044±0.001, Iron: 0.711±0.000, Copper: 0.833±0.082, Zinc: 0.580±0.001). The obtained result declared that...


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