Natural & Applied Sciences

Research Papers/Topics Natural & Applied Sciences

Enhancing Cowpea Production through Arbuscular Mycorrhizal Fungi Inoculation and Wide Interspecific Crosses

Abstract: Cowpea is a multipurpose legume crop that serves as human food, livestock fodder, and income source and is widely produced in sub-Saharan Africa. Soil fertility and attack by insect pests and diseases are significant limitations to its production. Although farm inputs such as phosphate-rich fertilizers and pesticides can solve the mentioned constraints, they are costly for resource-poor farmers. This study aimed at determining the effect of arbuscular mycorrhizal fungi inoculation ...

Assessment of Rodents’ Postharvest Losses in On-Farm Maize Storage in Kenya

Abstract: Rodents are one of the major postharvest pests that affect food security by impacting on both food availability and safety. However, knowledge of the impact of rodents in on-farm maize storage systems in Kenya is limited. A survey was conducted in 2014 with a total of 630 farmers spread across the six maize growing agro-ecological zones (AEZs) to assess postharvest losses magnitudes in on-farm maize storage systems in Kenya, and the contribution of rodents to the losses. Experiment...

Genome Sequence Of Tsetse Polydnavirus: Insights Into Symbiotic Virus Evolution

Abstract: Tsetse flies are the sole vectors of African trypanosomiasis in humans (sleeping sickness) and animals (nagana). The whole genome sequence of the tsetse fly Glossina morsitans morsitans revealed the presence of putative bracoviral sequences (n = 310) widely spread in the genome. These sequences are similar to those identified in parasitic braconid wasps. Bracoviruses (family Polydnaviridae) encode proteins that lower host immunity allowing for the development of parasitoid larvae i...

Gene Expression Profiling of Odorant Binding Proteins in the Tsetse fly Glossina brevipalpis

Abstract: Glossina brevipalpis is a vector of trypanosomes that cause Animal African Trypanosomiasis. Chemoreception in tsetse flies is critical for identifying larvipositioning sites, hosts and mates. Odorant binding proteins (OBPs) are thought to mediate insect chemoreception by shuttling odours to receptors on olfactory sensory neurons. Little is known about the roles of OBPs in tsetse fly chemoreception. The genome of G. brevipalpis has been sequenced and 28 OBPs identified. This study a...

Evaluation of Yield, Chemical Composition and Antimicrobial Activity of Essential Oils of Tagetes Minuta L. (Asteraceae) Against Selected Phytopathogens

Abstract: Phytopathogenic fungi and bacteria cause enormous crop yield losses worldwide. Over the years, management of plant pathogens has primarily relied on the use of synthetic chemical antimicrobials and development of disease resistant varieties. Exploration for biologically active compounds from plants with an aim of discovery and development of novel and eco-friendly biopesticides to combat current and emerging phytopathogens has received increased interest in the recent past. This st...

Management of Mycotoxigenic Fungi and Associated Mycotoxins in Maize by Use of Hermetic Storage

Abstract: Maize is a staple food crop in Kenya with about 90% of the rural households depending on it. However, the grain is vulnerable to degradation by mycotoxin producing fungi in the field and during storage. Mycotoxins produced by these fungi cause significant economic losses and deleterious health effects to humans and animals. Strategies such as storage of maize in hermetic bags have been developed to reduce post-harvest grain losses due to pest attack, fungal growth and mycotoxin con...

The complimentary role of wild habitats in crop pollination: a case study on the eggplant (solatium melongena l., solanaceae)

Abstract: In this study, carried out in Nguruman, southwestern Kenya, 10 bee species were identified as eggplant pollinators. Their foraging behaviour was shown to be affected by the prevailing ambient temperature and not time of the day or the number of flowers per square meter in the eggplant fields. Only 2 bee species, Xylocopa caffra and Narnia sp., visited the eggplant blossoms at a great frequency. Their pollinator effectiveness was determined in two eggplant cultivars (the Black Beaut...

Effects of Hermetic Bag Storage on Insect Pest Damage, Mould Infection and a Flatoxin Contamination on Maize Grain in Makueni County, Kenya

Abstract: For centuries, maize producers in sub-Saharan Africa have been plagued by post-harvest losses from insect infestation and mould infections, with small-scale producers representing the most vulnerable populations. Participatory on-farm trials were conducted to assess the effectiveness of triple layer hermetic (PICS™) bags for storage of maize in small-scale farmers’ stores in rural villages in Makueni County,Kenya. PICS bags are three-layer hermetic bag-system that forms a barri...

Rearing Method and Life History of Laboratory Bred African Coffee White Stem Borer, Monochamus Leuconotus (Pascoe) (Coleoptera: Cerambycidae)

Abstract: African coffee white stem borer, Monochamus leuconotus (Pascoe), Cerambycidae, is a major insect pest of coffee that causes yield losses as high as 25%. However, studying biology of Cerambycids is difficult due to the nature of their life cycle, which is mostly spent in the wood. This study aimed at developing and describing a rearing method of M. leuconotus using original artificial diet and its life history in the laboratory. The bioecology information generated will be useful in...

Comparative and Functional Analysis of Tsetse Fly Aquaporins

Abstract: Tsetse flies (Glossina sp) are the vector of African trypanosomes, the causative agents of sleeping sickness in man and Nagana in cattle. The insect is strictly hematophagous, taking in significantly high quantities of water during bloodmeals that present a considerable osmotic challenge. This necessitates machinery for eliminating excess water. One potential candidate is a group of membrane channel proteins called aquaporins (AQPs) that are responsible for the movement of water ac...

The Role of Social Capital in Malaria Control in Nyabondo, Western Kenya

Abstract: By 2015, target 8 of the United Nations Millennium Development Goal number six aimed to have halted and began to reverse the incidence of malaria and other major diseases. One of the strategies through which this target was expected to be achieved is to increase the proportion of the population in malaria risk areas who use effective malaria prevention and treatment measures. Malaria is one of the most common vector-borne diseases widespread in tropical and subtropical regions, inc...

Genetic Variation in Two Populations of the Brown Ear Tick, Rhipicephalus Appendiculatus, in Kenya

Abstract: Several studies have shown that Rhipicephalus appendiculatus from geographically isolated areas differ in their susceptibilities to Theileria parva, the haemoprotozoan causative agent of East Coast Fever. In Kenya, R appendiculatus from Muguga have been reported to be less susceptible to T. parva infection than those from Rusinga Islands. As the same tick species from different areas shows variation in vector competence, it was considered to be of epidemiological interest to determ...

Isolation and characterization of actinobacteria with potential for control of fusarium spp and colletotrichum kahawae

Abstract: Smallholder subsistence farming is important in the production of food crops for basic livelihoods and income generation in rural areas. However, yields have remained low due to high disease, weed and pest incidences. Currently, crop pests and diseases are largely controlled by use of chemicals, which have adverse effects on the environment and nontarget organisms. There is need for new methods that are environmentally safe to supplement existing control strategies in order to enha...

Biosorption of heavy metals using water hyacinth Eichhornia crassipes (Mart.) solms-Laubach: adsorption properties and technological assessment

Abstract: The adsorption of heavy metal ions (Cd2+), Cu2+), Pb2+) and Zn2+) by untreated water hyacinth Eichhornia crassipes obtained from Lake Victoria, Kenya was investigated under batch mode. A The effects of sample part, particle size, contact time, solution pH, and temperature, shaking speed, adsorbent dosage and initial metal ion concentration of aqueous solutions were investigated. The roots showed highest ability for the uptake of heavy metals (Cu, Zn, Cd and Pb) from aqueous solutio...

Study on the Biology and Some Ecological Aspects of Chilo partellus (Swinhoe) (Lepidoptera: Crambidae) in Relation to its Altitudinal Expansion

Abstract: Survey was carried out during 2004 main cropping season to investigate the abundance and distribution of stemborers, with emphasis on C. partellus in central and eastern Ethiopia. Three species of stemborers, Chilo partellus (Swinhoe), Busseola fusca (Fuller) and Sesamia calamistis (Hampson) were recorded. C. partellus was dominant and widely distributed species recorded in 96 % of the surveyed sites. On the other hand, B. fusca was mainly recorded in highland areas while S. calami...


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